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Literature summary extracted from

  • Yamasaki, Y.; Hayashi, I.; Ohta, Y.; Nakagawa, T.; Kawamukai, M.; Matsuda, H.
    Purification and mode of action of chitosanolytic enzyme from Enterobacter sp. G-1 (1993), Biosci. Biotechnol. Biochem., 57, 444-449.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 50000
-
 x * 50000, SDS-PAGE Enterobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Enterobacter sp.
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Enterobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Enterobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 5.84
-
 after Sephadex G-100 chromatography Enterobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Enterobacter sp. ?
-
 ?
3.2.1.132 chitosan + H2O hydrolysis of: colloidal chitosan 80% deacetylated, not 100% deacetylated Enterobacter sp. additional information GlcNAc4-6 and colloidal chitin are hydrolyzed to GlcNAc2 ?
3.2.1.132 chitosan + H2O hydrolysis of: colloidal chitosan 80% deacetylated, not 100% deacetylated Enterobacter sp. additional information the main cleavage site with GlcNAc3-6 is the second linkage from the non-reducing end, based on the pattern of pNp-GlcNAc2-5 ?
3.2.1.132 chitosan + H2O hydrolysis of: colloidal chitosan 80% deacetylated, not 100% deacetylated Enterobacter sp. additional information colloidal chitosan is hydrolyzed to GlcNAc2 and to similar partially N-acetylated chitooligosaccharides ?
3.2.1.132 colloidal chitin + H2O
-
 Enterobacter sp. ?
-
 ?
3.2.1.132 glycol chitin + H2O
-
 Enterobacter sp. ?
-
 ?
3.2.1.132 N-acetyl-chitooligosaccharides + H2O
-
 Enterobacter sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 50000, SDS-PAGE Enterobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 50
-
-
 Enterobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 20
-
 stable Enterobacter sp.