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Literature summary extracted from

  • Sakai, K.; Katsumi, R.; Isobe, A.; Nanjo, F.
    Purification and hydrolytic action of a chitosanase from Nocardia orientalis (1991), Biochim. Biophys. Acta, 1079, 65-72.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Amycolatopsis orientalis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Amycolatopsis orientalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Amycolatopsis orientalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.132 culture filtrate
-
 Amycolatopsis orientalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 61.4
-
 after Sephadex G-75 chromatography Amycolatopsis orientalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Amycolatopsis orientalis ?
-
 ?
3.2.1.132 chitosan + H2O enzyme is capable of cleaving between glucosamine and glucosamine or N-acetylglucosamine, but not cleaving between N-acetylglucosamine and glucosamine Amycolatopsis orientalis additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 chitosan + H2O the smallest of the substrates is a tetramer of glucosamine Amycolatopsis orientalis additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 chitosan + H2O most susceptible substrates are: chitohexaose and chitoheptaose Amycolatopsis orientalis additional information mixture of dimer and trimer of glucosamine ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 50
-
-
 Amycolatopsis orientalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 40
-
 15 min, stable up to Amycolatopsis orientalis
3.2.1.132 80
-
 15 min, 55% loss of activity Amycolatopsis orientalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5 6
-
 Amycolatopsis orientalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4.5 6.5 more than 80% of activity maximum at pH 4.5 and 6.5 Amycolatopsis orientalis