Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pelletier, A.; Sygusch, J.
    Purification and characterization of three chitosanase activities from Bacillus megaterium P1 (1990), Appl. Environ. Microbiol., 56, 844-848.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 chitosan substrate inhibition, isoform A Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 additional information 0.8 mg/ml chitosan Priestia megaterium
3.2.1.132 additional information
-
 additional information at pH 5.6, 22°C, Km value 0.8 mg per ml, isoform A Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 22000
-
 x * 22000, enzyme C, SDS-PAGE Priestia megaterium
3.2.1.132 43000
-
 x * 43000, enzyme B, SDS-PAGE Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Priestia megaterium
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Priestia megaterium
-
-
-
3.2.1.132 Priestia megaterium
-
 three isoforms, chitosanase A, 43 kilodalton, is highly specific for chitosan and represents the major chitosan-hydrolyzing species. Chitosanases B, 39.5 kilodalton, and C, 22 kilodalton, correspond to minor activities and possess comparable specific activities toward chitosan, chitin, and cellulose
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 3 enzymes: A, B and C Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 154.8
-
 isoform A, pH 5.6, 22°C Priestia megaterium
3.2.1.132 163.8
-
 after Mono-S cation exchange chromatography with 0.06 to 0.08 M KCl Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
3.2.1.132 -20°C, 50% glycerol, stable for at least 6 months Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 carboxymethylcellulose + H2O substrate for isoforms B, C Priestia megaterium ?
-
 ?
3.2.1.132 chitin + H2O substrate for isoform B, C Priestia megaterium ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Priestia megaterium ?
-
 ?
3.2.1.132 chitosan + H2O specific substrate, isoform A Priestia megaterium ?
-
 ?
3.2.1.132 chitosan + H2O enzyme A is highly specific for chitosan, enzyme B and C possess comparable specific activities towards chitosan, chitin and cellulose Priestia megaterium additional information glucosamine oligomers, predominantly dimers and trimers ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 43000, enzyme B, SDS-PAGE Priestia megaterium
3.2.1.132 ? x * 22000, enzyme C, SDS-PAGE Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 50
-
-
 Priestia megaterium
3.2.1.132 50
-
 enzyme A Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 45
-
 isoform A, stable up to Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4.5 6.5 isoform A Priestia megaterium