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Literature summary extracted from

  • Okajima, S.; Ando, A.; Shinoyama, H.; Fujii, T.
    Purification and characterization of an extracellular chitosanase produced by Amycolatopsis sp. CsO-2 (1994), J. Ferment. Bioeng., 77, 617-620.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Hg2+
-
 Amycolatopsis sp.
3.2.1.132 N-bromosuccinimide
-
 Amycolatopsis sp.
3.2.1.132 p-chloromercuribenzoate
-
 Amycolatopsis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 extracellular
-
 Amycolatopsis sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 27000
-
 gel filtration Amycolatopsis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Amycolatopsis sp.
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Amycolatopsis sp.
-
 CsO-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Amycolatopsis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Amycolatopsis sp. ?
-
 ?
3.2.1.132 chitosan + H2O most susceptible substrate is: 100% deacetylated chitosan Amycolatopsis sp. additional information
-
 ?
3.2.1.132 chitosan + H2O deacetylation degree from 70% to 100% Amycolatopsis sp. additional information
-
 ?
3.2.1.132 additional information no activity with chitin (i.e. 100% acetylated chitosan) Amycolatopsis sp. ?
-
 ?
3.2.1.132 additional information no activity with glucosamine dimer and trimer Amycolatopsis sp. ?
-
 ?
3.2.1.132 additional information degrades glucosamine tetramer to dimer and pentamer and to dimer and trimer Amycolatopsis sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 27000, SDS-PAGE Amycolatopsis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 55
-
 pH 5.3 Amycolatopsis sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132
-
-
 stable Amycolatopsis sp.
3.2.1.132 50
-
 60 min, stable Amycolatopsis sp.
3.2.1.132 60
-
 60 min, 40% loss of activity Amycolatopsis sp.
3.2.1.132 70
-
 60 min, 70% loss of activity Amycolatopsis sp.
3.2.1.132 80
-
 60 min, 80% loss of activity Amycolatopsis sp.
3.2.1.132 100
-
 10 min, 50% loss of activity Amycolatopsis sp.