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Literature summary extracted from

  • Tominaga, Y.; Tsujisaka, Y.
    Purification and some enzymatic properties of the chitosanase from Bacillus R-4 which lyses Rhizopus cell walls (1975), Biochim. Biophys. Acta, 410, 145-155.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.132 Chelating agents especially EDTA, activate Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Fe2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 Hg2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 L-cysteine
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 monoiodoacetate
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 Ni2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 p-chloromercuribenzoate
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 reduced glutathione
-
 Bacillus sp. (in: Bacteria)
3.2.1.132 sulfhydryl reagents glutathione or L-cysteine restores activity Bacillus sp. (in: Bacteria)
3.2.1.132 Zn2+
-
 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 31000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Bacillus sp. (in: Bacteria) R-4
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Bacillus sp. (in: Bacteria) K-1
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus sp. (in: Bacteria)
-
 K-1
-
3.2.1.132 Bacillus sp. (in: Bacteria)
-
 R-4
-
3.2.1.132 Bacillus sp. (in: Bacteria) K-1
-
 K-1
-
3.2.1.132 Bacillus sp. (in: Bacteria) R-4
-
 R-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 R-4 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 35.61
-
 after Sephadex G-75 chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) R-4 ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
3.2.1.132 chitosan + H2O hydrolysis of: glycol chitosan Bacillus sp. (in: Bacteria) additional information
-
 ?
3.2.1.132 chitosan + H2O hydrolysis of: glycol chitosan Bacillus sp. (in: Bacteria) R-4 additional information
-
 ?
3.2.1.132 chitosan + H2O hydrolysis of: glycol chitosan Bacillus sp. (in: Bacteria) K-1 additional information
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 40
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 50
-
 stable Bacillus sp. (in: Bacteria)
3.2.1.132 70
-
 15 min, 30% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.6
-
-
 Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Bacillus sp. (in: Bacteria) isoelectric focusing
-
 8.3