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Literature summary extracted from

  • Teplitsky, A.; Shulami, S.; Moryles, S.; Zaide, G.; Shoham, Y.; Shoham, G.
    Crystallization and preliminary X-ray analysis of alpha-D-glucuronidase from Bacillus stearothermophilus T-6 (1999), Acta Crystallogr. Sect. D, 55, 869-872.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.131 overexpression in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.131 two crystal forms, form T1 is obtained by the vapour-diffusion method using polyethylene glycol as precipitant and 2-propanol as organic additive, tetragonal space group P41212 or P43212 with a and b: 76.1 A and c: 331.2 A, form M1 at slightly lower pH and lower concentration of 2-propanol, monoclinic space group P21 with a: 65.8 A, b: 127.4 A, c: 96.6 A and beta: 97.9° Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.131 78480
-
 alpha2, 2 * 78480, deduced from nucleotide sequence Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.131 xylan + H2O Geobacillus stearothermophilus
-
 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
3.2.1.131 xylan + H2O Geobacillus stearothermophilus T-6
-
 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.131 Geobacillus stearothermophilus
-
 T-6
-
3.2.1.131 Geobacillus stearothermophilus T-6
-
 T-6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.131 xylan + H2O
-
 Geobacillus stearothermophilus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
3.2.1.131 xylan + H2O liberates 4-O-methyl-D-glucuronic acid from the main chain of the hardwood xylan Geobacillus stearothermophilus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
3.2.1.131 xylan + H2O
-
 Geobacillus stearothermophilus T-6 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
3.2.1.131 xylan + H2O liberates 4-O-methyl-D-glucuronic acid from the main chain of the hardwood xylan Geobacillus stearothermophilus T-6 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.131 dimer alpha2, 2 * 78480, deduced from nucleotide sequence Geobacillus stearothermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.131 Geobacillus stearothermophilus
-
-
 5.42