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Literature summary extracted from

  • Nurizzo, D.; Nagy, T.; Gilbert, H.J.; Davies, G.J.
    The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A (2002), Structure, 10, 547-556.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.131 expression as a His10-thioredoxin fusion protein in Escherichia coli Cellvibrio japonicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.131 vapor phase diffusion using the hanging drop method, 250 mM MgCl2 and 20% v/v ethylene glycol with 15% w/v polyethylene glycol 3350 as prcipitant at 20°C, triclinic space group P1 with a: 69.9 A, b: 74.7 A, c: 87.6 A, alpha: 115.2°, beta: 93.1° and gamma: 109.3° Cellvibrio japonicus
3.2.1.139 hanging-drop vapor diffusion method Cellvibrio japonicus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.131 D365A much less active than wild type Cellvibrio japonicus
3.2.1.131 D365C much less active than wild type Cellvibrio japonicus
3.2.1.131 E292A much less active than wild type Cellvibrio japonicus
3.2.1.131 E292C much less active than wild type Cellvibrio japonicus
3.2.1.131 E393A much less active than wild type Cellvibrio japonicus
3.2.1.131 E393C less active than wild type Cellvibrio japonicus
3.2.1.139 D365A activity is 0.0001% of wild-type activity Cellvibrio japonicus
3.2.1.139 D365C activity is 0.0001% of wild-type activity Cellvibrio japonicus
3.2.1.139 E292A activity is 0.0001% of wild-type activity Cellvibrio japonicus
3.2.1.139 E292C activity is 0.0001% of wild-type activity Cellvibrio japonicus
3.2.1.139 E393A activity is 0.0001% of wild-type activity Cellvibrio japonicus
3.2.1.139 E393C activity is22% of wild-type activity Cellvibrio japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.131 0.28
-
 aldotriouronic acid 37°C, pH 6.5 Cellvibrio japonicus
3.2.1.131 0.36
-
 aldotetraouronic acid 37°C, pH 6.5 Cellvibrio japonicus
3.2.1.131 0.42
-
 aldopentaouronic acid 37°C, pH 6.5 Cellvibrio japonicus
3.2.1.131 0.53
-
 aldobiouronic acid 37°C, pH 6.5 Cellvibrio japonicus
3.2.1.139 0.28
-
 aldotriouronic acid
-
 Cellvibrio japonicus
3.2.1.139 0.36
-
 aldotetraouronic acid
-
 Cellvibrio japonicus
3.2.1.139 0.42
-
 aldopentaouronic acid
-
 Cellvibrio japonicus
3.2.1.139 0.53
-
 aldobiouronic acid
-
 Cellvibrio japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.131 77000
-
 alpha2, 2 * 77000, SDS-PAGE, crystal structure Cellvibrio japonicus
3.2.1.131 150000
-
 gel filtration Cellvibrio japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.131 xylan + H2O Cellvibrio japonicus
-
 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.131 Cellvibrio japonicus
-
-
-
3.2.1.139 Cellvibrio japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.131
-
 Cellvibrio japonicus
3.2.1.139 recombinant Cellvibrio japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.131 aldobiouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
3.2.1.131 aldopentaouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
3.2.1.131 aldotetraouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
3.2.1.131 aldotriouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
3.2.1.131 xylan + H2O
-
 Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
3.2.1.131 xylan + H2O liberates 4-O-methyl-D-glucuronic acid from the main chain of the hardwood xylan Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
3.2.1.131 xylan + H2O
-
 Cellvibrio japonicus xylobiose + ?
-
 ?
3.2.1.139 aldobiouronic acid + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.2.1.139 aldopentaouronic acid + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.2.1.139 aldotetraouronic acid + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.2.1.139 aldotriouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.131 dimer alpha2, 2 * 77000, SDS-PAGE, crystal structure Cellvibrio japonicus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.139 GlcA67A
-
 Cellvibrio japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.131 69.2
-
 aldobiouronic acid 37°C, pH 6.5 Cellvibrio japonicus
3.2.1.131 96
-
 aldotriouronic acid 37°C, pH 6.5 Cellvibrio japonicus
3.2.1.131 103
-
 aldotetraouronic acid 37°C, pH 6.5 Cellvibrio japonicus
3.2.1.131 132
-
 aldopentaouronic acid 37°C, pH 6.5 Cellvibrio japonicus
3.2.1.139 69.2
-
 aldobiouronic acid
-
 Cellvibrio japonicus
3.2.1.139 96
-
 aldotriouronic acid
-
 Cellvibrio japonicus
3.2.1.139 102.5
-
 aldotetraouronic acid
-
 Cellvibrio japonicus
3.2.1.139 131.9
-
 aldopentaouronic acid
-
 Cellvibrio japonicus