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Literature summary extracted from

  • Leggate, D.R.; Bryant, J.M.; Redpath, M.B.; Head, D.; Taylor, P.W.; Luzio, J.P.
    Expression, mutagenesis and kinetic analysis of recombinant K1E endosialidase to define the site of proteolytic processing and requirements for catalysis (2002), Mol. Microbiol., 44, 749-760.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.129 expression in Escherichia coli as fusion protein Vectrevirus K1E

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.129 D138A lower Km than wild-type enzyme Vectrevirus K1E
3.2.1.129 D154A lower Km than wild-type enzyme Vectrevirus K1E
3.2.1.129 DELTA DI insoluble enzyme Vectrevirus K1E
3.2.1.129 DELTA DII insoluble enzyme Vectrevirus K1E
3.2.1.129 DELTA P-loop higher Km than wild-type enzyme Vectrevirus K1E
3.2.1.129 G153A less turnover than wild-type enzyme Vectrevirus K1E
3.2.1.129 Trunc N45 lower Km than wild-type enzyme Vectrevirus K1E
3.2.1.129 W159A insoluble enzyme Vectrevirus K1E

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.129 1 17.7 polysialic acid 37°C, pH 6.5, G153A Vectrevirus K1E
3.2.1.129 3.7
-
polysialic acid 37°C, pH 6.5, Trunc N45 Vectrevirus K1E
3.2.1.129 4.2 17.7 polysialic acid 37°C, pH 6.5, wild-type Vectrevirus K1E
3.2.1.129 6.4
-
polysialic acid 37°C, pH 6.5, D154A Vectrevirus K1E
3.2.1.129 8.3
-
polysialic acid 37°C, pH 6.5, D138A Vectrevirus K1E
3.2.1.129 27.1
-
polysialic acid 37°C, pH 6.5, DELTA P-loop Vectrevirus K1E

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.129 76000
-
x * 76000, SDS-PAGE Vectrevirus K1E

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.129 poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O Vectrevirus K1E
-
fragments of poly(sialic) acid or oligo(sialic) acids
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.129 Vectrevirus K1E
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.129
-
Vectrevirus K1E

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.129 poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O
-
Vectrevirus K1E fragments of poly(sialic) acid or oligo(sialic) acids
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.129 ? x * 76000, SDS-PAGE Vectrevirus K1E

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.129 0.01 1 polysialic acid 37°C, pH 6.5, G153A Vectrevirus K1E
3.2.1.129 0.2
-
polysialic acid 37°C, pH 6.5, D138A Vectrevirus K1E
3.2.1.129 0.4 1.5 polysialic acid 37°C, pH 6.5, wild-type Vectrevirus K1E
3.2.1.129 0.5
-
polysialic acid 37°C, pH 6.5, Trunc N45 Vectrevirus K1E
3.2.1.129 1.2
-
polysialic acid 37°C, pH 6.5, D154A Vectrevirus K1E
3.2.1.129 1.4
-
polysialic acid 37°C, pH 6.5, DELTA P-loop Vectrevirus K1E