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Literature summary extracted from

  • Hallenbeck, P.C.; Vimr, E.R.; Yu, F.; Bassler, B.; Troy, F.A.
    Purification and properties of a bacteriophage-induced endo-N-acetylneuraminidase specific for poly-alpha-2,8-sialosyl carbohydrate units (1987), J. Biol. Chem., 262, 3553-3561.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.129 0.0066
-
 polysialic acid pH 7.5, capsule isolated from Escherichia coli N67, soluble enzyme Escherichia phage K1F
3.2.1.129 0.051
-
 polysialic acid pH 7.5, capsule isolated from Escherichia coli K1, soluble enzyme Escherichia phage K1F
3.2.1.129 0.071
-
 polysialic acid pH 7.5, capsule isolated from Escherichia coli K1, phage-bound enzyme Escherichia phage K1F
3.2.1.129 1.2
-
 oligo(sialic) acid pH 7.5, isolated from Escherichia coli K1, phage-bound enzyme Escherichia phage K1F
3.2.1.129 1.6
-
 oligo(sialic) acid pH 7.5, from Escherichia coli K1, soluble enzyme Escherichia phage K1F

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.129 105000
-
 3 * 105000, SDS-PAGE Escherichia phage K1F
3.2.1.129 328000
-
 gel filtration Escherichia phage K1F

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.129 poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O Escherichia phage K1F
-
 fragments of poly(sialic) acid or oligo(sialic) acids
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.129 Escherichia phage K1F
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.129
-
 Escherichia phage K1F

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.129 4219
-
-
 Escherichia phage K1F

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.129 oligo(sialic) acid + H2O
-
 Escherichia phage K1F fragments of oligo(sialic) acid
-
 ?
3.2.1.129 poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O
-
 Escherichia phage K1F fragments of poly(sialic) acid or oligo(sialic) acids
-
 ?
3.2.1.129 poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O polysialic acid capsule isolated from Escherichia coli N67 or Escherichia coli K1 Escherichia phage K1F fragments of poly(sialic) acid or oligo(sialic) acids
-
 ?
3.2.1.129 poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O the minimum requirement for cleavage is alpha-2,8-(NeuAc)5 Escherichia phage K1F fragments of poly(sialic) acid or oligo(sialic) acids
-
 ?
3.2.1.129 poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O bacterial and neural membrane glycoconjugates Escherichia phage K1F fragments of poly(sialic) acid or oligo(sialic) acids
-
 ?
3.2.1.129 poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O oligosialic acid from Escherichia coli K1 Escherichia phage K1F fragments of poly(sialic) acid or oligo(sialic) acids
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.129 trimer 3 * 105000, SDS-PAGE Escherichia phage K1F