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Literature summary extracted from

  • Horibata, Y.; Okino, N.; Ichinose, S.; Omori, A.; Ito, M.
    Purification, characterization, and cDNA cloning of a novel acidic endoglycoceramidase from the jellyfish, Cyanea nozakii (2000), J. Biol. Chem., 275, 31297-31304.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.123 detergent required Cyanea nozakii
3.2.1.123 Lubrol Px optimal concentration: 0.4% Cyanea nozakii
3.2.1.123 Triton X-100 activates at 0.2% Cyanea nozakii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.123 EGCase and EGCase P Cyanea nozakii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.123 Hg2+ complete inhibition at 2 mM Cyanea nozakii
3.2.1.123 SDS complete inhibition at 0.1% Cyanea nozakii
3.2.1.123 taurodeoxycholate strong inhibitor Cyanea nozakii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.123 0.35
-
 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide pH 3, 37°C Cyanea nozakii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.123 51000
-
 gel filtration Cyanea nozakii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.123 Cyanea nozakii Q9GV16 jellyfish
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.123 glycoprotein enzyme contains 6 potential N-glycosylation sites Cyanea nozakii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.123 EGCase and EGCase P Cyanea nozakii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.123 2.52
-
-
 Cyanea nozakii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Cyanea nozakii Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD1b Cyanea nozakii Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O weak substrate Cyanea nozakii Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Cyanea nozakii Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 Galbeta(1-4)Glcbeta(1-1)ceramide + H2O weak substrate Cyanea nozakii Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. lactosylceramide Cyanea nozakii Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GT1b Cyanea nozakii NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Cyanea nozakii NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GQ1b Cyanea nozakii NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD3 Cyanea nozakii NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.123 ? x * 51000, SDS-PAGE Cyanea nozakii
3.2.1.123 monomer 1 * 51000, SDS-PAGE Cyanea nozakii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.123 3
-
-
 Cyanea nozakii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.123 Cyanea nozakii calculated from amino acid sequence
-
 5.61