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Literature summary extracted from

  • Ito, M.; Ikegami, Y.; Yamagata, T.
    Kinetics of endoglycoceramidase action toward cell-surface glycosphingolipids of erythrocytes (1993), Eur. J. Biochem., 218, 645-649.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.123 Activator protein increases the activity of EGCase II 468fold to 616fold Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.123 0.044
-
 NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide from horse erythrocyte cell surface, pH 7, 37°C Rhodococcus sp.
3.2.1.123 0.047
-
 GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide i.e. Gg3Cer from guinea pig erythrocyte cell surface, pH 7, 37°C Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.123 Rhodococcus sp.
-
 molecular species: II
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.123 GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. Gg3Cer Rhodococcus sp. GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
3.2.1.123 glycosphingolipid + H2O cell-surface glycosphingolipids of erythrocytes, horse-erythrocyte cell-surface GM3 and guinea pig erythrocyte cell-surface Gg3Cer Rhodococcus sp. ?
-
 ?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.123 5.5
-
-
 Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.123 7
-
 90% activity for NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide, 64% activity for Gg3Cer Rhodococcus sp.