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Literature summary extracted from

  • Ito, M.; Yamagata, T.
    A novel glycosphingolipid-degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids [published erratum appears in J Biol Chem 1987 Feb 5;262(4):1926] (1986), J. Biol. Chem., 261, 14278-14282.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.123 taurodeoxycholate 0.5 mg/ml, 2.5fold increase of activity Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.123 Cu2+
-
Rhodococcus sp.
3.2.1.123 Hg2+
-
Rhodococcus sp.
3.2.1.123 Zn2+
-
Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.123 Rhodococcus sp.
-
G-74-2
-
3.2.1.123 Rhodococcus sp. G-74-2
-
G-74-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.123
-
Rhodococcus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.123 culture supernatant
-
Rhodococcus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.123 additional information
-
-
Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.123 Fucalpha(1->3)GalNAcalpha(1->3)[Fucalpha(1->2)]Galbeta(1->4)Glcbeta(1-1)ceramide + H2O 84% of the activity with galactosylcerebroside Rhodococcus sp. Fucalpha(1->3)GalNAcalpha(1->3)[Fucalpha(1->2)]Galbeta(1->4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O as active as lactosylceramide Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. G-74-2 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O as active as lactosylceramide Rhodococcus sp. G-74-2 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O as active as lactosylceramide Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. lactosylceramide Rhodococcus sp. Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Galbeta(1-6)Galbeta(1-6)Galbeta(1-1)ceramide + H2O i.e. neogalactosylceramide, 77% of the activity with lactosylceramide Rhodococcus sp. Galbeta(1-6)Galbeta(1-6)Gal + ceramide
-
?
3.2.1.123 GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GM2, 94% of the activity with lactosylceramide Rhodococcus sp. GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)Glcbeta(1-1)ceramide + H2O 35% of the activity with lactosylceramide Rhodococcus sp. GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Manalpha(1-3)Manbeta(1-4)Glcbeta(1-1)ceramide + H2O 88% of the activity with lactosylceramide Rhodococcus sp. Manalpha(1-3)Manbeta(1-4)Glc + ceramide
-
?
3.2.1.123 Manbeta(1-4)Glcbeta(1-1)ceramide + H2O 93% of the activity with lactosylceramide Rhodococcus sp. Manbeta(1-4)Glc + ceramide
-
?
3.2.1.123 additional information no substrate: N-linked glycoproteins Rhodococcus sp. ?
-
?
3.2.1.123 additional information no substrate: O-linked glycoproteins Rhodococcus sp. ?
-
?
3.2.1.123 additional information hardly attacks linkages between monosaccharides and ceramides, i.e. cerebrosides, or between oligosaccharides and diacylglycerol, i.e. glycoglycerolipids Rhodococcus sp. ?
-
?
3.2.1.123 additional information no substrate: N-linked glycoproteins Rhodococcus sp. G-74-2 ?
-
?
3.2.1.123 additional information no substrate: O-linked glycoproteins Rhodococcus sp. G-74-2 ?
-
?
3.2.1.123 additional information hardly attacks linkages between monosaccharides and ceramides, i.e. cerebrosides, or between oligosaccharides and diacylglycerol, i.e. glycoglycerolipids Rhodococcus sp. G-74-2 ?
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD, 93% of the activity with lactosylceramide Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GT1b Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O 93% of the activity with lactosylceramide Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. sialylparagloboside, as active as lactosylceramide Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide
-
?
3.2.1.123 NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GT, 81% of the activity with lactosylceramide Rhodococcus sp. NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.123 6
-
-
Rhodococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.123 5 9 stable Rhodococcus sp.