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Literature summary extracted from

  • Pauly, M.; Andersen, L.N.; Kauppinen, S.; Kofod, L.V.; York, W.S.; Albersheim, P.; Darvill, A.
    A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme (1999), Glycobiology, 9, 93-100.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.120 expression in Aspergillus oryzae Aspergillus aculeatus
3.2.1.151
-
 Aspergillus aculeatus

General Stability

EC Number General Stability Organism
3.2.1.120 very stable below 35°C Aspergillus aculeatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 3.6
-
 xyloglucan pH 5, 37°C Aspergillus aculeatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.120 23600
-
 x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence Aspergillus aculeatus
3.2.1.120 23760
-
 x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence Aspergillus aculeatus
3.2.1.120 34000
-
 x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence Aspergillus aculeatus
3.2.1.151 23600
-
 mass spectrometry Aspergillus aculeatus
3.2.1.151 34000
-
 SDS-PAGE Aspergillus aculeatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.151 xyloglucan + H2O Aspergillus aculeatus
-
 xyloglucan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.120 Aspergillus aculeatus
-
-
-
3.2.1.151 Aspergillus aculeatus O94218
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.120 no glycoprotein no N-glycosylation site Aspergillus aculeatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.151 homogeneity Aspergillus aculeatus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.120 R1-Glcbeta(1-4)Glcbeta[Xylbeta(1-6)Glcbeta(1-4)](1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + H2O = R1-Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose stereoselective cleavage of substrate Aspergillus aculeatus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.120 260
-
 21°C, pH 5.0 Aspergillus aculeatus
3.2.1.151 260
-
-
 Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.120 xyloglucan fragment oligosaccharide + H2O
-
 Aspergillus aculeatus isoprimeverose
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Aspergillus aculeatus xyloglucan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.120 ? x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.120 50
-
 2 h, loss of 80% of activity Aspergillus aculeatus
3.2.1.151 30
-
 optimally stable below Aspergillus aculeatus
3.2.1.151 50
-
 80% loss of activity after 2h Aspergillus aculeatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 113
-
 xyloglucan from tamarind, pH 5, 37°C Aspergillus aculeatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.120 3 3.8
-
 Aspergillus aculeatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.151 3 3.8 optimally stable, stability declines sharply below pH 2.8 and above pH 5 Aspergillus aculeatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.151 Aspergillus aculeatus pH gradient 3.5-9.5
-
 3.4