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Literature summary extracted from

  • Matsumoto, T.; Sakai, F.; Hayashi, T.
    A xyloglucan-specific endo-1,4-beta-glucanase isolated from auxin-treated pea stems (1997), Plant Physiol., 114, 661-667.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 0.64
-
 xyloglucan pH 6.2, 35°C Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.120 70000
-
 gel filtration Pisum sativum
3.2.1.120 77000
-
 1 * 77000, SDS-PAGE Pisum sativum
3.2.1.151 70000
-
 gel filtration Pisum sativum
3.2.1.151 77000
-
 SDS-PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.151 xyloglucan + H2O Pisum sativum
-
 xyloglucan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.120 Pisum sativum
-
 auxin-treated
-
3.2.1.151 Pisum sativum
-
 auxin-treated pea, var. Alaska
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.120
-
 Pisum sativum
3.2.1.151 homogeneity Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.120 stem
-
 Pisum sativum
-
3.2.1.151 stem
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.120 1294
-
 pH 6.2, 35°C Pisum sativum
3.2.1.151 1.294
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.120 additional information no substrate: carboxymethylcellulose, swollen cellulose Pisum sativum ?
-
 ?
3.2.1.120 additional information no substrate: (1-3,1-4)-beta-glucan Pisum sativum ?
-
 ?
3.2.1.120 xyloglucan fragment oligosaccharide + H2O specifically cleaves 1,4-beta-glucosyl-linkages Pisum sativum isoprimeverose products are nona- and heptasaccharides ?
3.2.1.151 additional information no substrate: swollen cellulose Pisum sativum ?
-
 ?
3.2.1.151 additional information no substrate: (1--3,1--4)-beta-glucan Pisum sativum ?
-
 ?
3.2.1.151 additional information no substrate: carboxymethylcellulose Pisum sativum ?
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Pisum sativum xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O specifically cleaves the 1,4-beta-glucosyl linkages of the xyloglucan backbone to yield mainly nona- and heptasaccharides Pisum sativum xyloglucan oligosaccharides mainly nona- and heptasaccharides ?

Subunits

EC Number Subunits Comment Organism
3.2.1.120 monomer 1 * 77000, SDS-PAGE Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 35
-
-
 Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 6.2
-
-
 Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.151 4.5 8 active between pH 4.5 and pH 8 Pisum sativum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.120 Pisum sativum chromatofocusing
-
 8.1
3.2.1.151 Pisum sativum pH gradient 6-9
-
 8.1