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Literature summary extracted from

  • Kato, Y.; Matsushita, J.; Kubodera, T.; Matsuda, K.
    A novel enzyme producing isoprimeverose from oligoxyloglucans of Aspergillus oryzae (1985), J. Biochem., 97, 801-810.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.120 Cu2+
-
Aspergillus oryzae
3.2.1.120 Fe2+
-
Aspergillus oryzae
3.2.1.120 Fe3+
-
Aspergillus oryzae
3.2.1.120 Hg2+
-
Aspergillus oryzae
3.2.1.120 SDS
-
Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.120 115000
-
2 * 115000, SDS-PAGE Aspergillus oryzae
3.2.1.120 230000
-
gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.120 xyloglucan fragment oligosaccharide + H2O Aspergillus oryzae highly specific for isoprimeverose isoprimeverose is split off from the nonreducing end of the backbone of the substrate ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.120 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.120
-
Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.120 commercial preparation
-
Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.120 38.42
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.120 xyloglucan fragment oligosaccharide + H2O highly specific for Aspergillus oryzae isoprimeverose isoprimeverose is split off from the nonreducing end of the backbone of the substrate ?

Subunits

EC Number Subunits Comment Organism
3.2.1.120 dimer 2 * 115000, SDS-PAGE Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.120 60
-
-
Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.120 50
-
stable up to Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.120 5
-
-
Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.120 5 7 45°C, 10 min, stable Aspergillus oryzae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.120 Aspergillus oryzae isoelectric focusing
-
3.9