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Literature summary extracted from

  • Lizotte, P.A.; Poulton, J.E.
    Catabolism of cyanogenic glycosides by purified vicianin hydrolase from Squirrel's foot fern (Davallia trichomanoides Blume) (1988), Plant Physiol., 86, 322-324.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.119 Ag+ strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
3.2.1.119 castanospermine indolizidine alkaloid, strong, with (R)-prunazin as substrate Davallia trichomanoides
3.2.1.119 Cu2+ strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
3.2.1.119 delta-gluconolactone strong, with (R)-prunazin as substrate Davallia trichomanoides
3.2.1.119 diethyldithiocarbamate weak, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
3.2.1.119 Fe2+ weak, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
3.2.1.119 Hg2+ strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
3.2.1.119 additional information less than 10% inhibition by Ca2+, Zn2+, Fe3+, Co2+, Cd2+ and Pb2+, no inhibition by EDTA, 2,2'-dipyridyl, o-pheanthroline, 2-mercaptoethanol, dithiothreitol, iodacetic acid, iodoacetamide and N-methylmaleimide Davallia trichomanoides
3.2.1.119 p-chloromercuriphenylsulfonate strong, with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.119 3.2
-
 (R)-Prunasin 30°C, pH 6 Davallia trichomanoides
3.2.1.119 3.39
-
 p-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6 Davallia trichomanoides
3.2.1.119 4.9
-
 (R)-Vicianin 30°C, pH 6 Davallia trichomanoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.119 73000
-
 gel filtration Davallia trichomanoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.119 (R)-vicianin + H2O Davallia trichomanoides involved in catabolism of cyanogenic glycosides mandelonitrile + vicianose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.119 Davallia trichomanoides
-
-
-
3.2.1.119 Davallia trichomanoides
-
 Squirrel's foot fern, var. Blume
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.119 additional information no glycoprotein Davallia trichomanoides

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.119
-
 Davallia trichomanoides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.119 fiddlehead
-
 Davallia trichomanoides
-
3.2.1.119 frond young Davallia trichomanoides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.119 (R)-amygdalin + H2O reaction 27% of the rate of vicianine hydrolysis Davallia trichomanoides mandelonitrile + beta-gentiobiose
-
 ?
3.2.1.119 (R)-amygdalin + H2O
-
 Davallia trichomanoides (R)-mandelonitrile + beta-gentiobiose
-
 ?
3.2.1.119 (R)-prunasin + H2O reaction 14% of the rate of vicianine hydrolysis Davallia trichomanoides mandelonitrile + D-glucose
-
 ?
3.2.1.119 (R)-vicianin + H2O i.e. beta-vicianoside of (R)-mandelonitrile, strict requirement for (R)-epimers Davallia trichomanoides mandelonitrile + vicianose
-
 ?
3.2.1.119 (R)-vicianin + H2O cyanogenic disaccharides preferred over monosaccharides Davallia trichomanoides mandelonitrile + vicianose
-
 ?
3.2.1.119 (R)-vicianin + H2O involved in catabolism of cyanogenic glycosides Davallia trichomanoides mandelonitrile + vicianose
-
 ?
3.2.1.119 4-nitrophenyl beta-D-glucopyranoside + H2O poor substrate Davallia trichomanoides 4-nitrophenol + D-glucopyranose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.119 30
-
 assay at Davallia trichomanoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.119 5
-
 with p-nitrophenyl-beta-D-glucopyranoside as substrate Davallia trichomanoides
3.2.1.119 5.5
-
 with (R)-vicianin as substrate Davallia trichomanoides
3.2.1.119 6
-
 with (R)-prunazin as substrate Davallia trichomanoides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.119 Davallia trichomanoides
-
 4.7 4.6