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Literature summary extracted from

  • Zheng, L.; Poulton, J.E.
    Temporal and spatial expression of amygdalin hydrolase and (R)-(+)-mandelonitrile lyase in black cherry seeds (1995), Plant Physiol., 109, 31-39.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.117 AH I, two clones of near full length cDNA Prunus serotina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.117 60300
-
 AH I, calculated from known amino acid sequence Prunus serotina
3.2.1.117 62000
-
 SDS-PAGE Prunus serotina
3.2.1.117 62000
-
 AH I Prunus serotina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.117 (R)-amygdalin + H2O Prunus serotina first step of cyanogenesis from amygdalin (R)-prunasin + D-glucose
-
 ?
4.1.2.10 (R)-mandelonitrile Prunus serotina in Prunus serotina Ehrh. macerates, the cyanogenic diglucoside (R)-amygdalin undergoes stepwise degradation to HCN catalyzed by amygdalin hydrolase, prunasin hydrolase, and (R)-(+)-mandelonitrile lyase cyanide + benzaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.117 Prunus serotina
-
-
-
4.1.2.10 Prunus serotina P52706 Ehrh.
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.117 glycoprotein
-
 Prunus serotina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.117 seed procambial cells Prunus serotina
-
4.1.2.10 fruit temporal expression of amygdalin hydrolase and (R)-(+)-mandelonitrile lyase in ripening fruit. Expression may be under transcriptional control during fruit maturation. (R)-(+)-mandelonitrile lyase transcripts are localized within cotyledonary parenchyma cell Prunus serotina
-
4.1.2.10 seed
-
 Prunus serotina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.117 (R)-amygdalin + H2O
-
 Prunus serotina (R)-prunasin + D-glucose
-
 ?
3.2.1.117 (R)-amygdalin + H2O first step of cyanogenesis from amygdalin Prunus serotina (R)-prunasin + D-glucose
-
 ?
4.1.2.10 (R)-mandelonitrile in Prunus serotina Ehrh. macerates, the cyanogenic diglucoside (R)-amygdalin undergoes stepwise degradation to HCN catalyzed by amygdalin hydrolase, prunasin hydrolase, and (R)-(+)-mandelonitrile lyase Prunus serotina cyanide + benzaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 (R)-(+)-Mandelonitrile lyase
-
 Prunus serotina