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Literature summary extracted from

  • Li, C.P.; Swain, E.; Poulton, J.E.
    Prunus serotina amygdalin hydrolase and prunasin hydrolase. Purification, N-terminal sequencing, and antibody production (1992), Plant Physiol., 100, 282-290.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.117 1.65
-
 (R)-amygdalin AH II' Prunus serotina
3.2.1.117 1.88
-
 (R)-amygdalin AH II Prunus serotina
3.2.1.117 2.01
-
 (R)-amygdalin AH I Prunus serotina
3.2.1.117 2.2
-
 (R)-amygdalin AH I' Prunus serotina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.117 52000
-
 all isoforms, gel filtration Prunus serotina
3.2.1.117 60000
-
 all isoforms, SDS-PAGE Prunus serotina
3.2.1.118 68000
-
 PH IIb and PH I, native PAGE Prunus serotina
3.2.1.118 69500
-
 PH IIa, native PAGE Prunus serotina
3.2.1.118 140000
-
 PH IIa, native PAGE Prunus serotina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.117 (R)-amygdalin + H2O Prunus serotina first step of cyanogenesis from amygdalin (R)-prunasin + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O Prunus serotina involved in cyanogenesis from (R)-amygdalin mandelonitrile + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.117 Prunus serotina
-
 Ehrh., black cherry
-
3.2.1.118 Prunus serotina
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.117 glycoprotein
-
 Prunus serotina
3.2.1.118 glycoprotein
-
 Prunus serotina

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.117 four isoforms Prunus serotina
3.2.1.118
-
 Prunus serotina

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.117 (R)-amygdalin + H2O = (R)-prunasin + D-glucose four isoforms found, AH I, AH I', AH II, AH II' Prunus serotina
a
3.2.1.118 (R)-prunasin + H2O = D-glucose + mandelonitrile three isoforms found, PH I, PH IIa, PH IIb Prunus serotina
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.117 seed
-
 Prunus serotina
-
3.2.1.118 seed
-
 Prunus serotina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.117 0.45
-
 purified AH II' Prunus serotina
3.2.1.117 0.48
-
 purified AH I' and AH II Prunus serotina
3.2.1.117 0.58
-
 purified AH I Prunus serotina
3.2.1.118 2.8
-
 purified PH I Prunus serotina
3.2.1.118 4.9
-
 purified PH II Prunus serotina

Storage Stability

EC Number Storage Stability Organism
3.2.1.117 4°C, 10 mM histidine-HCl buffer, pH 6.0 Prunus serotina
3.2.1.118 -20°C lyophylized powder Prunus serotina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.117 (R)-amygdalin + H2O
-
 Prunus serotina (R)-prunasin + D-glucose
-
 ?
3.2.1.117 (R)-amygdalin + H2O first step of cyanogenesis from amygdalin Prunus serotina (R)-prunasin + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O
-
 Prunus serotina D-glucose + mandelonitrile
-
 ?
3.2.1.118 (R)-prunasin + H2O involved in cyanogenesis from (R)-amygdalin Prunus serotina mandelonitrile + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.117 monomer 1 * 60000, SDS-PAGE Prunus serotina
3.2.1.118 dimer 2 * 69500, SDS-PAGE Prunus serotina
3.2.1.118 monomer 1 * 68000, SDS-PAGE, PH I and PH IIb Prunus serotina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.117 4.5 5.5 all isoforms Prunus serotina