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Literature summary extracted from

  • Mitsuishi,Y.; Kobayashi, M.; Matsuda, K.
    Dextran alpha-1,2 debranching enzyme from Flavobacterium sp. M-73: Its production and purification (1979), Agric. Biol. Chem., 43, 2283-2290.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.115 additional information effect of different carbon sources on enzyme activity, inducibility of enzyme Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.115 125000
-
 x * 125000, SDS-PAGE Flavobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.115 additional information Flavobacterium sp. hydrolysis of alpha-1,2-glucosidic linkages at branching points of dextran ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.115 Flavobacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.115 2750fold Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.115 66
-
 pH 5.6, 40°C Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.115 dextran + H2O B-1299, soluble, major linkages: alpha-1,6-, alpha-1,3-, alpha-1,2-, most effective substrate for induction of enzyme Flavobacterium sp. alpha-D-glucose hydrolysis of 1,2-alpha-D-glucosidic linkages at the branched points, producing free D-glucose ?
3.2.1.115 dextran + H2O clinical dextran, major linkages: alpha-1,6-, alpha-1,3-, 55% as effective as soluble dextran for induction of enzyme Flavobacterium sp. alpha-D-glucose hydrolysis of 1,2-alpha-D-glucosidic linkages at the branched points, producing free D-glucose ?
3.2.1.115 additional information effect of different carbon sources on enzyme activity, no activity with alpha-1,3-glucan, starch, glucose or sucrose Flavobacterium sp. ?
-
 ?
3.2.1.115 additional information hydrolysis of alpha-1,2-glucosidic linkages at branching points of dextran Flavobacterium sp. ?
-
 ?
3.2.1.115 mutan IG-1 + H2O major linkages: alpha-1,3-, alpha-1,6-, 18% as effective as soluble dextran for induction of enzyme Flavobacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.115 ? x * 125000, SDS-PAGE Flavobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.115 40
-
 assay at Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.115 5.6
-
 assay at Flavobacterium sp.