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Literature summary extracted from

  • Kawar, Z.; Karaveg, K.; Moremen, K.W.; Jarvis, D.L.
    Insect cells encode a class II alpha-mannosidase with unique properties (2001), J. Biol. Chem., 276, 16335-16340.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.114 alpha-mannosidase III cDNA is cloned Spodoptera frugiperda

General Stability

EC Number General Stability Organism
3.2.1.24 glutathione S-transferase/mannosidase III fusion protein is inactivated by freezing Spodoptera frugiperda
3.2.1.114 freezing inactivates column-bound glutathione S-transferase-tagged secreted form of SfManIII Spodoptera frugiperda

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 EDTA 1 mM, more than 90% inactivation Spodoptera frugiperda
3.2.1.24 swainsonine IC50: 10 nM Spodoptera frugiperda
3.2.1.24 ZnCl2 92% inhibition by 1 mM, 56% inhibition by 0.01 mM Spodoptera frugiperda
3.2.1.114 EDTA alpha-mannosidase III, 1 mM, 90% inhibition of 4-nitrophenyl alpha-D-mannoside hydrolysis, 5 mM, complete inhibition of (Man)5(GlcNAc)2-PA hydrolysis Spodoptera frugiperda
3.2.1.114 swainsonine alpha-mannosidase III, IC50: 10 nM Spodoptera frugiperda
3.2.1.114 Zn2+ alpha-mannosidase III Spodoptera frugiperda

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 Golgi apparatus
-
 Spodoptera frugiperda 5794
-
3.2.1.114 Golgi membrane alpha-mannosidase III, integral membrane glycoprotein with type II topology Spodoptera frugiperda 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Co2+ 0.1 mM enhances activity 30.2fold Spodoptera frugiperda
3.2.1.24 Mn2+ 1 mM enhances activity 6fold Spodoptera frugiperda
3.2.1.24 Ni2+ 0.1 mM, enhances activity to 119% of control Spodoptera frugiperda
3.2.1.114 Co2+ activation Spodoptera frugiperda
3.2.1.114 Co2+ alpha-mannosidase III, cobalt-dependent Spodoptera frugiperda
3.2.1.114 Mn2+ alpha-mannosidase III, activates to a lesser extend than Co2+ Spodoptera frugiperda
3.2.1.114 Ni2+ alpha-mannosidase III, little activation Spodoptera frugiperda

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.114 150000
-
 x * 150000, intact glutathione S-transferase-tagged secreted form of SfManIII, SDS-PAGE Spodoptera frugiperda

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.114 additional information Spodoptera frugiperda alpha-mannosidase III probably provides an alternate N-glycan processing pathway in Sf-9 cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Spodoptera frugiperda
-
-
-
3.2.1.114 Spodoptera frugiperda
-
 Sf-9, alpha-mannosidase III
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.114 glycoprotein integral membrane glycoprotein Spodoptera frugiperda

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 glutathione S-transferase/mannosidase III fusion protein Spodoptera frugiperda
3.2.1.114 glutathione S-transferase-tagged secreted form of SfManIII Spodoptera frugiperda

Storage Stability

EC Number Storage Stability Organism
3.2.1.24 4°C, glutathione S-transferase/mannosidase III fusion protein is stable for at least 1 week Spodoptera frugiperda
3.2.1.114 4°C, column-bound glutathione S-transferase-tagged secreted form of SfManIII, at least 1 week, stable Spodoptera frugiperda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 Man5GlcNAc2 + 2 H2O
-
 Spodoptera frugiperda Man3GlcNAc2 + 2 mannose
-
 ?
3.2.1.24 Man9GlcNAc2 + H2O
-
 Spodoptera frugiperda Man8GlcNAc2 + mannose
-
 ?
3.2.1.24 additional information no hydrolysis of GlcNAcMan5GlcNAc2 Spodoptera frugiperda ?
-
 ?
3.2.1.114 4-nitrophenyl alpha-D-mannoside + H2O alpha-mannosidase III Spodoptera frugiperda 4-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.114 additional information alpha-mannosidase III hydrolyzes (Man)5-9(GlcNAc)2, not: GlcNAc(Man)5(GlcNAc)2 Spodoptera frugiperda ?
-
 ?
3.2.1.114 additional information alpha-mannosidase III probably provides an alternate N-glycan processing pathway in Sf-9 cells Spodoptera frugiperda ?
-
 ?
3.2.1.114 pyridylaminated (Man)5(GlcNAc)2 + H2O via a (Man)4(GlcNAc)2 intermediate Spodoptera frugiperda pyridylaminated (Man)3(GlcNAc)2 + alpha-D-mannose
-
 ?
3.2.1.114 pyridylaminated (Man)5(GlcNAc)2 + H2O alpha-mannosidase III Spodoptera frugiperda pyridylaminated (Man)3(GlcNAc)2 + alpha-D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.24 More SDS-PAGE reveals a major protein of 150000 Da and a minor protein with a MW of 130000 Da Spodoptera frugiperda
3.2.1.114 ? x * 150000, intact glutathione S-transferase-tagged secreted form of SfManIII, SDS-PAGE Spodoptera frugiperda

Synonyms

EC Number Synonyms Comment Organism
3.2.1.24 alpha-mannosidase III
-
 Spodoptera frugiperda

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.114 37
-
 assay at Spodoptera frugiperda

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 6.5 6.7
-
 Spodoptera frugiperda
3.2.1.114 6.5 6.7
-
 Spodoptera frugiperda

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.24 5.7 6.7 pH 5.7: about 35% of maximal activity, pH 6.5-6.7: optimum Spodoptera frugiperda
3.2.1.114 5.5 6.7 alpha-mannosidase III activity is negligible at pH 5.5, increases at pH 5.7-5.9, decreases at pH 6.1 and rises to a plateau at pH 6.5-6.7 Spodoptera frugiperda

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.24 0.00001
-
 IC50: 10 nM Spodoptera frugiperda swainsonine
3.2.1.114 0.00001
-
 alpha-mannosidase III, IC50: 10 nM Spodoptera frugiperda swainsonine