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Literature summary extracted from

  • Kaushal, G.P.; Szumilo, T.; Pastuszak, I.; Elbein, A.D.
    Purification to homogeneity and properties of mannosidase II from mung bean seedlings (1990), Biochemistry, 29, 2168-2176.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.114 Triton X-100 25-30% activation, 0.05% Vigna radiata

General Stability

EC Number General Stability Organism
3.2.1.114 glycerol stabilizes Vigna radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.114 1,4-Dideoxy-1,4-imino-D-mannitol much less effective than swainsonine Vigna radiata
3.2.1.114 alpha-1,6-linked trisaccharide of mannose 1.5 mM, 40% inhibition Vigna radiata
3.2.1.114 Cu2+ 0.2 mM CuCl2, 75% inhibition Vigna radiata
3.2.1.114 additional information not inhibited by Co2+, Zn2+; not inhibited by deoxymannojirimycin; not inhibited by EDTA; not inhibited by EGTA, 6-epicastanospermine, 2-episwainsonine, a trisaccharide of mannose residues linked in alpha-1,2-linkages, a tetrasaccharide of mannose with the nonreducing mannose linked in alpha-1,3-linkage to an alpha-1,2-linked trisaccharide of mannose; not inhibited by Mn2+, Mg2+ Vigna radiata
3.2.1.114 swainsonine i.e. (1S,2R,8R,8aR)-trihydroxyindolizidine; very strong Vigna radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.114 microsome
-
Vigna radiata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.114 additional information no metal ion requirement Vigna radiata
3.2.1.114 additional information not activated by Ca2+ Vigna radiata
3.2.1.114 additional information not activated by Zn2+ Vigna radiata
3.2.1.114 additional information not activated by Co2+ Vigna radiata
3.2.1.114 additional information not activated by Mg2+, Mn2+ Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.114 125000
-
x * 125000, SDS-PAGE Vigna radiata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.114 Vigna radiata
-
mung bean
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.114 glycoprotein presumably an N-linked glycoprotein with high-mannose chains Vigna radiata

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.114 1200fold Vigna radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.114 seedling
-
Vigna radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.114 additional information
-
-
Vigna radiata

Storage Stability

EC Number Storage Stability Organism
3.2.1.114 0-4°C, 10 mM HEPES buffer, pH 7.2, 10% glycerol, several days, stable Vigna radiata
3.2.1.114 frozen, 10 mM HEPES buffer, pH 7.1, 0.1% Triton X-100, 0.5 mM PMSF, 0.2 mM dithiothreitol, 10% glycerol, at least 2 months, stable Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 (Man)3GlcNAc + H2O poor substrate Vigna radiata ?
-
?
3.2.1.114 4-methylumbelliferyl alpha-D-mannoside + H2O
-
Vigna radiata 4-methylumbelliferone + alpha-D-mannose
-
?
3.2.1.114 4-nitrophenyl alpha-D-mannoside + H2O
-
Vigna radiata 4-nitrophenol + alpha-D-mannose
-
?
3.2.1.114 GlcNAc(Man)5GlcNAc + 2 H2O excellent substrate, removes the alpha-1,3- and alpha-1,6-mannosyl residues with preference for the alpha-1,6-linkage Vigna radiata GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose final product, via GlcNAc(Man)4GlcNAc ?
3.2.1.114 GlcNAc(Man)5GlcNAc + 2 H2O excellent substrate, removes the alpha-1,3- and alpha-1,6-mannosyl residues with preference for the alpha-1,6-linkage Vigna radiata GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose product structure: GlcNAcManalpha(1-3)(Manalpha(1-6))ManbetaGlcNAc ?
3.2.1.114 additional information not: Glc3(Man)9GlcNAc Vigna radiata ?
-
?
3.2.1.114 additional information not: (Man)9GlcNAc Vigna radiata ?
-
?
3.2.1.114 additional information not: (Man)5GlcNAc Vigna radiata ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.114 ? x * 125000, SDS-PAGE Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.114 6
-
-
Vigna radiata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.114 4.8 7 about half-maximal activity at pH 4.8 and 7 Vigna radiata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.114 6
-
below, high loss of activity within 1 week Vigna radiata