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Literature summary extracted from

  • Bonay, P.; Fresno, M.
    Isolation and purification of a neutral alpha(1,2)-mannosidase from Trypanosoma cruzi (1999), Glycobiology, 9, 423-433.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1-deoxymannojirimycin not significantly Trypanosoma cruzi
3.2.1.113 D-mannono-delta-lactam
-
 Trypanosoma cruzi
3.2.1.113 D-mannono-gamma-lactone
-
 Trypanosoma cruzi
3.2.1.113 EDTA strong Trypanosoma cruzi
3.2.1.113 Fe2+ strong Trypanosoma cruzi
3.2.1.113 kifunensine
-
 Trypanosoma cruzi
3.2.1.113 swainsonine
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.113 0.087
-
 p-nitrophenyl-alpha-D-mannoside 37°C, pH 6.1 Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.113 Co2+ activation Trypanosoma cruzi
3.2.1.113 Ni2+ activation Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.113 76000
-
 2 * 76000, SDS-PAGE Trypanosoma cruzi
3.2.1.113 150000 160000 gel filtration Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Trypanosoma cruzi
-
 intracellular protozoan, causes Chagas’ disease in humans, epimastigote stage
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113 partial Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Trypanosoma cruzi (Man)5GlcNAc + 4 D-mannose product is Man6GlcNAc ?
3.2.1.113 additional information no hydrolysis of alpha1,3-linkages Trypanosoma cruzi ?
-
 ?
3.2.1.113 additional information no hydrolysis of alpha1,6-linkages Trypanosoma cruzi ?
-
 ?
3.2.1.113 p-nitrophenyl-alpha-D-mannoside + H2O
-
 Trypanosoma cruzi 4-nitrophenol + D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.113 dimer 2 * 76000, SDS-PAGE Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 5.9 6.5
-
 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.113 0.0001
-
 swainsonine pH 6.1, 37°C Trypanosoma cruzi
3.2.1.113 0.002
-
 D-mannono-delta-lactam pH 6.1, 37°C Trypanosoma cruzi
3.2.1.113 0.006
-
 kifunensine pH 6.1, 37°C Trypanosoma cruzi