Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hamagashira, N.; Oku, H.; Mega, T.; Hase, S.
    Purification and characterization of hen oviduct alpha 1,2-mannosidase (1996), J. Biochem., 119, 998-1003.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.209 1-deoxymannojirimycin
-
 Gallus gallus
3.2.1.209 EDTA complete inactivation at 1.7 mM, Ca2+ restores Gallus gallus
3.2.1.209 additional information no inhibition by swainsonine Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.209 42000
-
 1 * 42000, SDS-PAGE Gallus gallus
3.2.1.209 50000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.209 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.209
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.209 oviduct
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.209 4000
-
 37°C, pH 7.0 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.209 alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine + 3 H2O
-
 Gallus gallus alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-2-pyridylamine + 3 D-mannopyranose
-
 ?
3.2.1.209 additional information no hydrolysis of alpha1,3-linkages Gallus gallus ?
-
 ?
3.2.1.209 additional information no hydrolysis of alpha1,6-linkages Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.209 monomer 1 * 42000, SDS-PAGE Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.209 6.5
-
-
 Gallus gallus