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Literature summary extracted from

  • Shah, N.; Kuntz, D.A.; Rose, D.R.
    Comparison of kifunensine and 1-deoxymannojirimycin binding to class I and II alpha-mannosidases demonstrates different saccharide distortions in inverting and retaining catalytic mechanisms (2003), Biochemistry, 42, 13812-13816.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.113 in complex with kifunensine Homo sapiens
3.2.1.114 crystal structure of enzyme with bound inhibitors kifunensine, swainsonine and 1-deoxymannojirimycin Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.114 1-deoxymannojirimycin more inhibitory than kifunensine, mode of binding Drosophila melanogaster
3.2.1.114 kifunensine weak, mode of binding Drosophila melanogaster
3.2.1.114 swainsonine mode of binding; very strong Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.114 Golgi apparatus
-
 Drosophila melanogaster 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.114 Zn2+ active site coordination to Zn2+ Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.114 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O Drosophila melanogaster N-glycosylation pathway Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Homo sapiens
-
-
-
3.2.1.114 Drosophila melanogaster
-
 PDB: 1PS3
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.114 Man5GlcNAc3-[protein] + 2 H2O = Man3GlcNAc3-[protein] + 2 alpha-D-mannopyranose catalytic mechanism Drosophila melanogaster
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 4-nitrophenyl alpha-D-mannoside + H2O
-
 Drosophila melanogaster 4-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.114 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O N-glycosylation pathway Drosophila melanogaster Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.114 More member of glycosyl hydrolase family 38 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.114 37
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.114 5.8
-
-
 Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.114 0.0000105
-
 swainsonine pH 5.75, 37°C Drosophila melanogaster
3.2.1.114 5.2
-
 kifunensine pH 5.75, 37°C Drosophila melanogaster