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Literature summary extracted from

  • Kimura, Y.; Yamaguchi, O.; Suehisa, H.; Tagaki, S.
    In vitro hydrolysis of oligomannose-type sugar chains by an alpha-1,2-mannosidase from microsomes of developing castor bean cotyledons (1991), Biochim. Biophys. Acta, 1075, 6-11.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1-deoxymannojirimycin
-
 Ricinus communis
3.2.1.113 Cd2+ strong Ricinus communis
3.2.1.113 Cu2+ strong Ricinus communis
3.2.1.113 EDTA
-
 Ricinus communis
3.2.1.113 Fe3+ strong Ricinus communis
3.2.1.113 Hg2+ strong Ricinus communis
3.2.1.113 additional information
-
 Ricinus communis
3.2.1.113 Sn2+ strong Ricinus communis
3.2.1.113 Ti4+ strong Ricinus communis
3.2.1.113 Tris
-
 Ricinus communis
3.2.1.113 Zn2+
-
 Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.113 0.26
-
 Man6GlcNAc 40°C, pH 5.5 Ricinus communis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.113 Ca2+ slight activation Ricinus communis
3.2.1.113 Ca2+ activation Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Ricinus communis
-
 castor bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113 partial Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.113 cotyledon
-
 Ricinus communis
-
3.2.1.113 seedling
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.113 0.0000036
-
 40°C, pH 5.5 Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 Man6GlcNAc + H2O
-
 Ricinus communis Man5GlcNAc + D-mannose
-
 ?
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O pyridylamino derivative of Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-3)ManbetaGlcNAc2 Ricinus communis (Man)5(GlcNAc)2 + D-mannose i.e. pyridylamino derivative of (Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc ?
3.2.1.113 additional information no substrate: (Man)4-(GlcNAc)2 Ricinus communis ?
-
 ?
3.2.1.113 pyridylamino derivative of (Man)9(GlcNAc)2 + H2O
-
 Ricinus communis pyridylamino derivative of (Man)5(GlcNAc)2 + D-mannose after 120 h incubation, via specific intermediates: overview ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 40
-
 assay at Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 5.5 6
-
 Ricinus communis