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Literature summary extracted from

  • Tulsiani, D.R.P.; Touster, O.
    Mannosidase IA from rat liver Golgi membranes (1989), Methods Enzymol., 179, 446-451.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.113 Triton X-100 requirement Rattus norvegicus
3.2.1.113 Triton X-100 0.12%, no activation at 0.07% and below Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1-deoxymannojirimycin reversible Rattus norvegicus
3.2.1.113 Co2+
-
 Rattus norvegicus
3.2.1.113 Cu2+
-
 Rattus norvegicus
3.2.1.113 EDTA
-
 Rattus norvegicus
3.2.1.113 additional information no inhibition by Ca2+; no inhibition by Mg2+ Rattus norvegicus
3.2.1.113 p-chloromercuriphenylsulfonic acid
-
 Rattus norvegicus
3.2.1.113 Tris
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.113 Golgi membrane
-
 Rattus norvegicus 139
-
3.2.1.113 membrane
-
 Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.113 230000
-
 PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Rattus norvegicus
-
 (Man)5GlcNAc + 4 D-mannose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.113 glycoprotein 0.88% hexoses Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113 mannosidase IA Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.113 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.113 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.2.1.113 0-4°C, in 10 mM potassium phosphate buffer, pH 7.2, 0.5% Triton X-100, 4-6 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Rattus norvegicus (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O the terminal mannose on the middle antenna appears to be the most susceptible residue Rattus norvegicus (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
-
 Rattus norvegicus ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
-
 Rattus norvegicus ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
-
 Rattus norvegicus ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O without alpha-1,2-linked mannose residues: no substrate Rattus norvegicus (Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.113 tetramer 4 * 57000-58000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 6
-
-
 Rattus norvegicus