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Literature summary extracted from

  • Kobata, A.; Amano, J.
    alpha-Mannosidases I and II from Aspergillus saitoi (1987), Methods Enzymol., 138, 779-785.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.113 1.25
-
 Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
 Aspergillus phoenicis
3.2.1.114 1.25
-
 Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc NaBH4 reduced Aspergillus phoenicis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.113 divalent cations not Aspergillus phoenicis
3.2.1.114 Ca2+ strongly activates, 1 mM, 13fold Aspergillus phoenicis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Aspergillus phoenicis
-
-
-
3.2.1.114 Aspergillus phoenicis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113
-
 Aspergillus phoenicis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.113 culture medium commercially available under the name of Morushin Aspergillus phoenicis
-
3.2.1.113 mycelium commercially available under the name of Morushin Aspergillus phoenicis
-
3.2.1.114 mycelium freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin Aspergillus phoenicis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.113 34.6
-
 37°C, pH 5.0 Aspergillus phoenicis
3.2.1.114 38000
-
-
 Aspergillus phoenicis

Storage Stability

EC Number Storage Stability Organism
3.2.1.113 -20°C, in 10 mM sodium acetate buffer, pH 5, with or without 1 mM CaCl2, at least 4 months Aspergillus phoenicis
3.2.1.113 4°C, in 10 mM sodium acetate buffer, pH 5, with or without 1 mM CaCl2, at least 4 months Aspergillus phoenicis
3.2.1.114 -20°C or 4°C, 10 mM sodium acetate buffer, pH 5, with or without CaCl2, 4 months, stable Aspergillus phoenicis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 (Manalpha(1-2))nManalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3)) Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Aspergillus phoenicis Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAc beta1-4GlcNAc + D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
-
 Aspergillus phoenicis ? + D-mannose
-
 ?
3.2.1.113 additional information no hydrolysis of alpha1,3-linkages Aspergillus phoenicis ?
-
 ?
3.2.1.113 additional information no hydrolysis of alpha1,6-linkages Aspergillus phoenicis ?
-
 ?
3.2.1.114 D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O NaBH4 reduced, poor substrate Aspergillus phoenicis ?
-
 ?
3.2.1.114 D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O NaBH4 reduced, good substrate Aspergillus phoenicis D-Manbeta(1-4)GlcNAc + alpha-D-mannose
-
 ?
3.2.1.114 Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Aspergillus phoenicis Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
-
 ?
3.2.1.114 additional information not: 4-nitrophenyl alpha-mannoside Aspergillus phoenicis ?
-
 ?
3.2.1.114 additional information not: Manalpha(1-6)(R-Manalpha(1-3))Manbeta(1-4)GlcNAc Aspergillus phoenicis ?
-
 ?
3.2.1.114 R-Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
-
 Aspergillus phoenicis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 37
-
 assay at Aspergillus phoenicis
3.2.1.114 37
-
 assay at Aspergillus phoenicis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 5
-
-
 Aspergillus phoenicis
3.2.1.114 5
-
-
 Aspergillus phoenicis