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Literature summary extracted from

  • Forsee, W.T.
    Characterization of microsomal and cytosolic alpha-1,2-mannosidases from mung bean hypocotyls (1985), Arch. Biochem. Biophys., 242, 48-57.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.114 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose Vigna radiata var. radiata
3.2.1.114 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose Vigna radiata var. radiata
3.2.1.114 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose Vigna radiata var. radiata
3.2.1.114 Cu2+ strong Vigna radiata var. radiata
3.2.1.114 EDTA Ca2+ restores Vigna radiata var. radiata
3.2.1.114 Mannosyl-mannose disaccharide in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose Vigna radiata var. radiata
3.2.1.114 Zn2+ strong Vigna radiata var. radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.114 cytosol 2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one Vigna radiata var. radiata 5829
-
3.2.1.114 membrane 2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one Vigna radiata var. radiata 16020
-
3.2.1.114 microsome 2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one Vigna radiata var. radiata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.114 Ca2+ activation Vigna radiata var. radiata
3.2.1.114 Ca2+ microsomal enzyme, activation Vigna radiata var. radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.114 39000
-
 cytosolic enzyme, gel filtration Vigna radiata var. radiata
3.2.1.114 460000
-
 microsomal enzyme, gel filtration, presumably in a micelle or aggregate containing detergent, phospholipid or other protein Vigna radiata var. radiata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.114 Vigna radiata var. radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.114 partial Vigna radiata var. radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.114 hypocotyl
-
 Vigna radiata var. radiata
-
3.2.1.114 seedling
-
 Vigna radiata var. radiata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc Vigna radiata var. radiata (Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
 ?
3.2.1.114 additional information no hydrolysis of alpha1,6-linkages Vigna radiata var. radiata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.114 More not identical with aryl-alpha-mannosidase Vigna radiata var. radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.114 37
-
 assay at Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.114 5.5 6
-
 Vigna radiata var. radiata