BRENDA - Enzyme Database

Characterization of microsomal and cytosolic alpha-1,2-mannosidases from mung bean hypocotyls

Forsee, W.T.; Arch. Biochem. Biophys. 242, 48-57 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.114
2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
Vigna radiata var. radiata
3.2.1.114
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
Vigna radiata var. radiata
3.2.1.114
6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
Vigna radiata var. radiata
3.2.1.114
Cu2+
strong
Vigna radiata var. radiata
3.2.1.114
EDTA
Ca2+ restores
Vigna radiata var. radiata
3.2.1.114
Mannosyl-mannose disaccharide
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
Vigna radiata var. radiata
3.2.1.114
Zn2+
strong
Vigna radiata var. radiata
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.114
cytosol
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
Vigna radiata var. radiata
5829
-
3.2.1.114
membrane
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
Vigna radiata var. radiata
16020
-
3.2.1.114
microsome
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
Vigna radiata var. radiata
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.114
Ca2+
activation; microsomal enzyme, activation
Vigna radiata var. radiata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.114
39000
-
cytosolic enzyme, gel filtration
Vigna radiata var. radiata
3.2.1.114
460000
-
microsomal enzyme, gel filtration, presumably in a micelle or aggregate containing detergent, phospholipid or other protein
Vigna radiata var. radiata
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.114
Vigna radiata var. radiata
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.114
partial
Vigna radiata var. radiata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.114
hypocotyl
-
Vigna radiata var. radiata
-
3.2.1.114
seedling
-
Vigna radiata var. radiata
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.114
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc
646575
Vigna radiata var. radiata
(Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
646575
Vigna radiata var. radiata
?
3.2.1.114
additional information
no hydrolysis of alpha1,6-linkages
646575
Vigna radiata var. radiata
?
-
-
-
-
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.114
More
not identical with aryl-alpha-mannosidase
Vigna radiata var. radiata
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.114
37
-
assay at
Vigna radiata var. radiata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.114
5.5
6
-
Vigna radiata var. radiata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.114
2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
Vigna radiata var. radiata
3.2.1.114
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
Vigna radiata var. radiata
3.2.1.114
6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
Vigna radiata var. radiata
3.2.1.114
Cu2+
strong
Vigna radiata var. radiata
3.2.1.114
EDTA
Ca2+ restores
Vigna radiata var. radiata
3.2.1.114
Mannosyl-mannose disaccharide
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
Vigna radiata var. radiata
3.2.1.114
Zn2+
strong
Vigna radiata var. radiata
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.114
cytosol
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
Vigna radiata var. radiata
5829
-
3.2.1.114
membrane
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
Vigna radiata var. radiata
16020
-
3.2.1.114
microsome
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
Vigna radiata var. radiata
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.114
Ca2+
activation; microsomal enzyme, activation
Vigna radiata var. radiata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.114
39000
-
cytosolic enzyme, gel filtration
Vigna radiata var. radiata
3.2.1.114
460000
-
microsomal enzyme, gel filtration, presumably in a micelle or aggregate containing detergent, phospholipid or other protein
Vigna radiata var. radiata
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.114
partial
Vigna radiata var. radiata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.114
hypocotyl
-
Vigna radiata var. radiata
-
3.2.1.114
seedling
-
Vigna radiata var. radiata
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.114
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc
646575
Vigna radiata var. radiata
(Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
646575
Vigna radiata var. radiata
?
3.2.1.114
additional information
no hydrolysis of alpha1,6-linkages
646575
Vigna radiata var. radiata
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.114
37
-
assay at
Vigna radiata var. radiata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.114
5.5
6
-
Vigna radiata var. radiata