Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Forsee, W.T.; Springfield, J.D.; Schutzbach, J.S.
    Effect of phospholipids on alpha-1,2-mannosidase activity (1982), J. Biol. Chem., 257, 9963-9967.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.113 additional information no activation by negatively charged phospholipids, dibutyryl-phosphocholine Oryctolagus cuniculus
3.2.1.113 phosphatidylcholine activation, with acyl-chains of different lengths, above C-4 Oryctolagus cuniculus
3.2.1.113 phosphatidylethanolamine activation, in the presence of 3.5 mM Triton X-100 Oryctolagus cuniculus
3.2.1.113 Triton X-100 activation together with zwitterionic phospholipids Oryctolagus cuniculus
3.2.1.113 Zwitterionic phospholipids activation, required for solubilized and purified enzyme, independent of acyl chain length or degree of saturation, an ordered lipid structure of either micelles or bilayers, mixed micelles together with Triton X-100 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.113 membrane
-
 Oryctolagus cuniculus 16020
-
3.2.1.113 microsome
-
 Oryctolagus cuniculus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113 partial, solubilization requires non-ionic detergents Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.113 liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc Oryctolagus cuniculus (Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 5.5 6.5 in the presence of egg yolk lyso-phosphatidylcholine Oryctolagus cuniculus
3.2.1.113 6.5
-
 in the presence of soybean phosphatidylinositol Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.113 5.2 7.3 about half-maximal activity at pH 5.2 and 7.3, in the presence of egg yolk lyso-phosphatidylcholine Oryctolagus cuniculus
3.2.1.113 5.5 7.5 about half-maximal activity at pH 5.5 and 7.5, in the presence of soybean phosphatidylinositol Oryctolagus cuniculus