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Literature summary extracted from

  • Imbert, M.J.; Glasgow, L.R.; Pizzo, S.V.
    Purification of an almond emulsin fucosidase on Cibacron blue-Sepharose and demonstration of its activity toward fucose-containing glycoproteins (1982), J. Biol. Chem., 257, 8205-8210.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.111 decreasing ionic strength inactivates Prunus dulcis
3.2.1.111 freeze-thawing results in 30% loss of initial activity Prunus dulcis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.111 Hg2+ strong Prunus dulcis
3.2.1.111 L-fucose
-
 Prunus dulcis
3.2.1.111 malonate above 20 mM Prunus dulcis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.111 Ca2+ slight activation Prunus dulcis
3.2.1.111 Mg2+ slight activation Prunus dulcis
3.2.1.111 additional information no activation by Mn2+ Prunus dulcis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.111 73000
-
 gel filtration Prunus dulcis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.111 Prunus dulcis
-
 almond
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.111 affinity chromatography on Blue Sepharose Prunus dulcis
3.2.1.111 partial Prunus dulcis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.111 emulsin commercially available Prunus dulcis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.111 0.0645
-
-
 Prunus dulcis

Storage Stability

EC Number Storage Stability Organism
3.2.1.111 4°C, less than 10% loss of activity within 6 months Prunus dulcis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.111 asialoorosomucoid + H2O specifically hydrolyzes fucose in alpha-1,3-linkage to N-acetylglucosamine of Asn-linked oligosaccharide chain branches Prunus dulcis ?
-
 ?
3.2.1.111 lactoferrin + H2O hydrolyzes only alpha-1,3-linkages between fucose and N-acetylglucosamine Prunus dulcis ?
-
 ?
3.2.1.111 additional information no substrates are alpha2-macroglobulin (MW 72600), bovine IgG glycopeptides or milk oligosaccharide 2'-fucosyllactose Prunus dulcis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.111 37
-
 assay at Prunus dulcis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.111 5.3
-
-
 Prunus dulcis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.111 4.3 5.8 about half-maximal activity at pH 4.3 and about 80% of maximal activity at pH 5.8 Prunus dulcis