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Literature summary extracted from

  • Cheng, T.C.; Harvey, S.P.; Stroup, A.N.
    Purification and properties of a highly active organophosphorus acid anhydrolase from Alteromonas undina (1993), Appl. Environ. Microbiol., 59, 3138-3140.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.8.2 2-mercaptoethanol activation, less effective than DTT Pseudoalteromonas undina
3.1.8.2 DTT activation Pseudoalteromonas undina

General Stability

EC Number General Stability Organism
3.1.8.2 2-mercaptoethanol enhances stability Pseudoalteromonas undina
3.1.8.2 DTT enhances stability Pseudoalteromonas undina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.8.2 EGTA Mn2+ reverses and stimulates over pre-EGTA-treatment activity level, Co2+ partially restores; reversible by Mn2+ or Co2+; strong Pseudoalteromonas undina
3.1.8.2 Fe2+ weak Pseudoalteromonas undina
3.1.8.2 iodoacetic acid
-
 Pseudoalteromonas undina
3.1.8.2 N-ethylmaleimide
-
 Pseudoalteromonas undina
3.1.8.2 Ni2+ weak Pseudoalteromonas undina
3.1.8.2 Zn2+
-
 Pseudoalteromonas undina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.8.2 Mn2+ activation Pseudoalteromonas undina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.8.2 53000
-
 1 * 53000 SDS-PAGE Pseudoalteromonas undina

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.2 Pseudoalteromonas undina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.2
-
 Pseudoalteromonas undina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.8.2 327.8
-
-
 Pseudoalteromonas undina
3.1.8.2 575
-
-
 Pseudoalteromonas undina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.2 4-nitrophenyl-ethyl(phenyl)phosphinate + H2O i.e. 4-nitrophenylethyl phenylphosphinate Pseudoalteromonas undina ?
-
 ?
3.1.8.2 4-nitrophenyl-ethyl(phenyl)phosphinate + H2O at 46% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
 ?
3.1.8.2 4-nitrophenyl-methyl(phenyl)phosphinate + H2O i.e. 4-nitrophenylmethyl phenylphosphinate Pseudoalteromonas undina ?
-
 ?
3.1.8.2 4-nitrophenyl-methyl(phenyl)phosphinate + H2O at 28% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
 ?
3.1.8.2 4-nitrophenylisopropyl phenylphosphinate + H2O at 22% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
 ?
3.1.8.2 4-nitrophenylpropyl phenylphosphinate + H2O at 24% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
 ?
3.1.8.2 diisopropyl fluorophosphate + H2O at about 30% of O-pinacolylmethylphosphonofluoridate hydrolysis Pseudoalteromonas undina diisopropyl phosphate + fluoride
-
 ?
3.1.8.2 diisopropyl fluorophosphate + H2O i.e. DFP Pseudoalteromonas undina diisopropyl phosphate + fluoride
-
 ?
3.1.8.2 mipafox + H2O i.e. N,N'-diisopropyl phosphorodiamidofluoridate, poor substrate Pseudoalteromonas undina ?
-
 ?
3.1.8.2 additional information not: 4-nitrophenylphosphate Pseudoalteromonas undina ?
-
 ?
3.1.8.2 additional information 4-nitrophenylacetate, bis(4-nitrophenyl)phosphate, tris(4-nitrophenyl)phosphate Pseudoalteromonas undina ?
-
 ?
3.1.8.2 O-cyclohexylmethylphosphonofluoridate + H2O at 114% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
 ?
3.1.8.2 O-pinacolylmethylphosphonofluoridate + H2O best substrate Pseudoalteromonas undina ?
-
 ?
3.1.8.2 paraoxon + H2O i.e. diethyl-4-nitrophenyl phosphate, poor substrate Pseudoalteromonas undina diethylphosphate + 4-nitrophenol
-
 ?
3.1.8.2 sarin + H2O at 38% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
 ?
3.1.8.2 tabun + H2O i.e. N,N-dimethylethylphosphoramidocyanidate, at 124% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.8.2 monomer 1 * 53000 SDS-PAGE Pseudoalteromonas undina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.8.2 25
-
 assay at Pseudoalteromonas undina
3.1.8.2 55
-
-
 Pseudoalteromonas undina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.8.2 917
-
 diisopropyl fluorophosphate value above Pseudoalteromonas undina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.8.2 8
-
-
 Pseudoalteromonas undina