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Literature summary extracted from

  • DeFrank, J.J.; Cheng, T.C.
    Purification and properties of an organophosphorus acid anhydrase from a halophilic bacterial isolate (1991), J. Bacteriol., 173, 1938-1943.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.8.2 2-mercaptoethanol activation, less effective than DTT Alteromonas sp.
3.1.8.2 DTT activation Alteromonas sp.

General Stability

EC Number General Stability Organism
3.1.8.2 DTT enhances stability Alteromonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.8.2 Ca2+
-
 Alteromonas sp.
3.1.8.2 EGTA only slightly by Fe3+ or Ni2+, not by Cs2+, Cu2+, Mg2+, Ca2+ or Zn2+; reversible by Mn2+ or Co2+ Alteromonas sp.
3.1.8.2 iodoacetamide
-
 Alteromonas sp.
3.1.8.2 Mipafox reversible Alteromonas sp.
3.1.8.2 N-ethylmaleimide
-
 Alteromonas sp.
3.1.8.2 PCMB
-
 Alteromonas sp.
3.1.8.2 Zn2+
-
 Alteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.8.2 additional information
-
 additional information
-
 Alteromonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.8.2 Mn2+ activation Alteromonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.8.2 60000
-
 1 * 60000, SDS-PAGE, under reducing and nonreducing conditions Alteromonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.2 Alteromonas sp.
-
 JD6.5, halophilic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.2
-
 Alteromonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.8.2 268
-
-
 Alteromonas sp.

Storage Stability

EC Number Storage Stability Organism
3.1.8.2 -70°C, in the presence of DTT, several months Alteromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.2 4-nitrophenyl-ethyl(phenyl)phosphinate + H2O 40% the rate of diisopropyl fluorophosphate hydrolysis Alteromonas sp. ?
-
 ?
3.1.8.2 4-nitrophenyl-methyl(phenyl)phosphinate + H2O at 49% the rate of diisopropyl fluorophosphate hydrolysis Alteromonas sp. ?
-
 ?
3.1.8.2 diisopropyl fluorophosphate + H2O i.e. DFP Alteromonas sp. diisopropyl phosphate + fluoride
-
 ?
3.1.8.2 additional information not: 4-nitrophenylphosphonate, 4-nitrophenylphosphinorylcholine Alteromonas sp. ?
-
 ?
3.1.8.2 additional information not: 4-nitrophenylphosphate Alteromonas sp. ?
-
 ?
3.1.8.2 additional information 4-nitrophenylacetate, bis(4-nitrophenyl)phosphate, tris(4-nitrophenyl)phosphate Alteromonas sp. ?
-
 ?
3.1.8.2 sarin + H2O at 20% the rate of diisopropyl fluorophosphate hydrolysis Alteromonas sp. ?
-
 ?
3.1.8.2 soman + H2O at 240% the rate of diisopropyl fluorophosphate hydrolysis Alteromonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.8.2 monomer 1 * 60000, SDS-PAGE, under reducing and nonreducing conditions Alteromonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.8.2 50
-
 3 mM diisopropyl fluorophosphate, pH 7.2, 1 mM MnCl2 Alteromonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.8.2 583
-
 diisopropyl fluorophosphate pH 7.5, 22°C Alteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.8.2 8.5
-
 diisopropyl fluorophosphate Alteromonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.8.2 7.6 9 about half-maximal activity at pH 7.6 and about 60% of maximal activity at pH 9 Alteromonas sp.