BRENDA - Enzyme Database

Glycosylphosphatidylinositol-phospholipase D: a tool for glycosylphosphatidylinositol structural analysis

Deeg, M.A.; Davitz, M.A.; Methods Enzymol. 250, 630-640 (1995)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.50
Nonidet P-40
stimulation
Bos taurus
3.1.4.50
Nonidet P-40
stimulation
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.50
overview
Bos taurus
3.1.4.50
overview
Homo sapiens
General Stability
EC Number
General Stability
Organism
3.1.4.50
stable over a range of 0.01-0.1% detergent, ionic detergents such as SDS are inhibitory, and bicarbonate buffer is inhibitory
Homo sapiens
3.1.4.50
stable over a range of 0.01-0.1% detergent, ionic detergents such as SDS are inhibitory, and bicarbonate buffer is inhibitory
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.50
1,10-phenanthroline
-
Bos taurus
3.1.4.50
1,10-phenanthroline
-
Homo sapiens
3.1.4.50
Bicarbonate buffer
-
Bos taurus
3.1.4.50
Bicarbonate buffer
-
Homo sapiens
3.1.4.50
EGTA
-
Bos taurus
3.1.4.50
EGTA
-
Homo sapiens
3.1.4.50
lipid A
-
Bos taurus
3.1.4.50
lipid A
-
Homo sapiens
3.1.4.50
lysophosphatidic acid
-
Bos taurus
3.1.4.50
lysophosphatidic acid
-
Homo sapiens
3.1.4.50
phosphatidic acid
-
Bos taurus
3.1.4.50
phosphatidic acid
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.50
Ca2+
activation
Bos taurus
3.1.4.50
Ca2+
activation
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.50
100000
-
gel filtration
Bos taurus
3.1.4.50
110000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.50
Bos taurus
-
-
-
3.1.4.50
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.50
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.50
plasma
-
Bos taurus
-
3.1.4.50
plasma
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.50
additional information
no substrate: phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol derived from HNO2-cleaved mfVSG, overview on substrate specificity
646476
Homo sapiens
?
-
-
-
-
3.1.4.50
additional information
no substrate: phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol derived from HNO2-cleaved mfVSG, overview on substrate specificity
646476
Bos taurus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.50
monomer
1 * 110000
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.50
7.4
-
assay at
Bos taurus
3.1.4.50
7.4
-
assay at
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.50
5
8
-
Bos taurus
3.1.4.50
5
8
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.50
Nonidet P-40
stimulation
Bos taurus
3.1.4.50
Nonidet P-40
stimulation
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.50
overview
Bos taurus
3.1.4.50
overview
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.50
stable over a range of 0.01-0.1% detergent, ionic detergents such as SDS are inhibitory, and bicarbonate buffer is inhibitory
Homo sapiens
3.1.4.50
stable over a range of 0.01-0.1% detergent, ionic detergents such as SDS are inhibitory, and bicarbonate buffer is inhibitory
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.50
1,10-phenanthroline
-
Bos taurus
3.1.4.50
1,10-phenanthroline
-
Homo sapiens
3.1.4.50
Bicarbonate buffer
-
Bos taurus
3.1.4.50
Bicarbonate buffer
-
Homo sapiens
3.1.4.50
EGTA
-
Bos taurus
3.1.4.50
EGTA
-
Homo sapiens
3.1.4.50
lipid A
-
Bos taurus
3.1.4.50
lipid A
-
Homo sapiens
3.1.4.50
lysophosphatidic acid
-
Bos taurus
3.1.4.50
lysophosphatidic acid
-
Homo sapiens
3.1.4.50
phosphatidic acid
-
Bos taurus
3.1.4.50
phosphatidic acid
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.50
Ca2+
activation
Bos taurus
3.1.4.50
Ca2+
activation
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.50
100000
-
gel filtration
Bos taurus
3.1.4.50
110000
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.50
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.50
plasma
-
Bos taurus
-
3.1.4.50
plasma
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.50
additional information
no substrate: phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol derived from HNO2-cleaved mfVSG, overview on substrate specificity
646476
Homo sapiens
?
-
-
-
-
3.1.4.50
additional information
no substrate: phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol derived from HNO2-cleaved mfVSG, overview on substrate specificity
646476
Bos taurus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.50
monomer
1 * 110000
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.50
7.4
-
assay at
Bos taurus
3.1.4.50
7.4
-
assay at
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.50
5
8
-
Bos taurus
3.1.4.50
5
8
-
Homo sapiens