BRENDA - Enzyme Database

Enzymatic properties of phosphatidylinositol-glycan-specific phospholipase C from rat liver and phosphatidylinositol-glycan-specific phospholipase D from rat serum [published erratum appears in Eur J Biochem 1991 Sep 15;200(3):812]

Stieger, S.; Diem, S.; Jakob, A.; Brodbeck, U.; Eur. J. Biochem. 197, 67-73 (1991)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.50
additional information
higher activity at low ionic strength, increasing amounts of NaCl, KCl, Na2SO4 or sodium phosphate decrease activity
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.50
1,10-phenanthroline
Ca2+ does not protect
Rattus norvegicus
3.1.4.50
2,2'-dipyridyl
Ca2+ does not protect
Rattus norvegicus
3.1.4.50
Ca2+
slight decrease of activity above 1 mM, activation at 0.0001-0.01 mM
Rattus norvegicus
3.1.4.50
additional information
no inhibition by 1,7-phenanthroline or 2,2'-dipyridyl
Rattus norvegicus
3.1.4.50
NaHCO3
kinetics, pH-dependent: at pH 7.5, not at pH 5.5, reversible
Rattus norvegicus
3.1.4.50
NaOCN
kinetics, pH-dependent, reversible
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.50
0.0001
-
phosphatidylinositol-glycan-anchor of membrane form of acetylcholinesterase
37°C, pH 7.4
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.50
soluble
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.50
Ca2+
activation; large increase of activity between 0.0001 and 0.01 mM, slight decrease above 1 mM
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.50
phosphatidylinositol-glycan-anchored membrane form of acetylcholinesterase + H2O
Rattus norvegicus
isolated from bovine erythrocyte membranes
soluble acetylcholinesterase + phosphatidic acid
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.50
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.50
serum
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.50
phosphatidylinositol-glycan-anchored membrane form of acetylcholinesterase + H2O
isolated from bovine erythrocyte membranes
646474
Rattus norvegicus
soluble acetylcholinesterase + phosphatidic acid
-
646474
Rattus norvegicus
-
3.1.4.50
phosphatidylinositol-glycan-anchored membrane form of acetylcholinesterase + H2O
isolated from bovine erythrocyte membranes
646474
Rattus norvegicus
soluble acetylcholinesterase + phosphatidic acid
-
646474
Rattus norvegicus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.50
6
-
higher activity at low ionic strength, increasing amounts of NaCl, KCl, Na2SO4 or sodium phosphate decrease activity
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.50
5
7
about 60% of maximal activity at pH 5 and about half-maximal activity at pH 7, no activity above pH 8.5
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.50
additional information
higher activity at low ionic strength, increasing amounts of NaCl, KCl, Na2SO4 or sodium phosphate decrease activity
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.50
1,10-phenanthroline
Ca2+ does not protect
Rattus norvegicus
3.1.4.50
2,2'-dipyridyl
Ca2+ does not protect
Rattus norvegicus
3.1.4.50
Ca2+
slight decrease of activity above 1 mM, activation at 0.0001-0.01 mM
Rattus norvegicus
3.1.4.50
additional information
no inhibition by 1,7-phenanthroline or 2,2'-dipyridyl
Rattus norvegicus
3.1.4.50
NaHCO3
kinetics, pH-dependent: at pH 7.5, not at pH 5.5, reversible
Rattus norvegicus
3.1.4.50
NaOCN
kinetics, pH-dependent, reversible
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.50
0.0001
-
phosphatidylinositol-glycan-anchor of membrane form of acetylcholinesterase
37°C, pH 7.4
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.50
soluble
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.50
Ca2+
activation; large increase of activity between 0.0001 and 0.01 mM, slight decrease above 1 mM
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.50
phosphatidylinositol-glycan-anchored membrane form of acetylcholinesterase + H2O
Rattus norvegicus
isolated from bovine erythrocyte membranes
soluble acetylcholinesterase + phosphatidic acid
-
Rattus norvegicus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.50
serum
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.50
phosphatidylinositol-glycan-anchored membrane form of acetylcholinesterase + H2O
isolated from bovine erythrocyte membranes
646474
Rattus norvegicus
soluble acetylcholinesterase + phosphatidic acid
-
646474
Rattus norvegicus
-
3.1.4.50
phosphatidylinositol-glycan-anchored membrane form of acetylcholinesterase + H2O
isolated from bovine erythrocyte membranes
646474
Rattus norvegicus
soluble acetylcholinesterase + phosphatidic acid
-
646474
Rattus norvegicus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.50
6
-
higher activity at low ionic strength, increasing amounts of NaCl, KCl, Na2SO4 or sodium phosphate decrease activity
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.50
5
7
about 60% of maximal activity at pH 5 and about half-maximal activity at pH 7, no activity above pH 8.5
Rattus norvegicus