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Literature summary extracted from

  • Stieger, S.; Diem, S.; Jakob, A.; Brodbeck, U.
    Enzymatic properties of phosphatidylinositol-glycan-specific phospholipase C from rat liver and phosphatidylinositol-glycan-specific phospholipase D from rat serum [published erratum appears in Eur J Biochem 1991 Sep 15;200(3):812] (1991), Eur. J. Biochem., 197, 67-73.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.50 additional information higher activity at low ionic strength, increasing amounts of NaCl, KCl, Na2SO4 or sodium phosphate decrease activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.50 1,10-phenanthroline Ca2+ does not protect Rattus norvegicus
3.1.4.50 2,2'-dipyridyl Ca2+ does not protect Rattus norvegicus
3.1.4.50 Ca2+ slight decrease of activity above 1 mM, activation at 0.0001-0.01 mM Rattus norvegicus
3.1.4.50 additional information no inhibition by 1,7-phenanthroline or 2,2'-dipyridyl Rattus norvegicus
3.1.4.50 NaHCO3 kinetics, pH-dependent: at pH 7.5, not at pH 5.5, reversible Rattus norvegicus
3.1.4.50 NaOCN kinetics, pH-dependent, reversible Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.50 0.0001
-
 phosphatidylinositol-glycan-anchor of membrane form of acetylcholinesterase 37°C, pH 7.4 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.50 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.50 Ca2+ activation Rattus norvegicus
3.1.4.50 Ca2+ large increase of activity between 0.0001 and 0.01 mM, slight decrease above 1 mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.50 phosphatidylinositol-glycan-anchored membrane form of acetylcholinesterase + H2O Rattus norvegicus isolated from bovine erythrocyte membranes soluble acetylcholinesterase + phosphatidic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.50 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.50 serum
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.50 phosphatidylinositol-glycan-anchor of membrane form of acetylcholinesterase + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.4.50 phosphatidylinositol-glycan-anchored membrane form of acetylcholinesterase + H2O isolated from bovine erythrocyte membranes Rattus norvegicus soluble acetylcholinesterase + phosphatidic acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.50 6
-
 higher activity at low ionic strength, increasing amounts of NaCl, KCl, Na2SO4 or sodium phosphate decrease activity Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.50 5 7 about 60% of maximal activity at pH 5 and about half-maximal activity at pH 7, no activity above pH 8.5 Rattus norvegicus