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Literature summary extracted from
- Characterization and partial purification of a novel mannosylphosphodolichol phosphodiesterase from chicken liver microsomes (1987), Eur. J. Biochem., 170, 363-368.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.49 | 2-mercaptoethanol | requirement, or other SH-reducing agents | Gallus gallus |  |
| 3.1.4.49 | Emulgen 909 | requirement, optimum concentration: 0.4% v/v | Gallus gallus | |
| 3.1.4.49 | additional information | no requirement for any phospholipid | Gallus gallus | |
| 3.1.4.49 | SH-reducing agents | e.g. 2-mercaptoethanol, absolute requirement | Gallus gallus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.4.49 | a heat-stable factor, precipitable by trichloroacetic acid, insoluble in lipid solvents, stabilizes, separation from this factor by ion-exchange chromatography inactivates irreversibly | Gallus gallus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.49 | bis(4-nitrophenyl)phosphate | 1 mM, competitive inhibition | Gallus gallus | |
| 3.1.4.49 | dolichol | 0.1 mM, strong, competitive inhibition | Gallus gallus | |
| 3.1.4.49 | dolichyl phosphate | 0.1 mM, strong, competitive inhibition | Gallus gallus | |
| 3.1.4.49 | additional information | not inhibited by D-mannose, mannose 1-phosphate, GDP, GMP, ADP, AMP, phosphate, diphosphate GDPmannose, UDP-N-acetylglucosamine, UDPglucose, UDPglucuronic acid, 4-nitrophenyl phosphate, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside or 4-nitrophenyl-beta-D-glucopyranoside | Gallus gallus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.49 | 0.00043 | - |
Mannosylphosphodolichol | pH 5.3, 37°C | Gallus gallus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.49 | microsome | - |
Rattus norvegicus | - |
- |
| 3.1.4.49 | microsome | predominantly | Gallus gallus | - |
- |
| 3.1.4.49 | mitochondrion | - |
Gallus gallus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.49 | CaCl2 | greatly stimulates, optimum concentration: 2 mM | Gallus gallus | |
| 3.1.4.49 | additional information | not activated by other divalent cations than Ca2+ | Gallus gallus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.49 | mannosylphosphodolichol + H2O | Gallus gallus | enzyme may be capable of degrading Dol-P-Man in vivo | D-mannose + dolichyl phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.49 | Gallus gallus | - |
- |
- |
| 3.1.4.49 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.49 | partial, 57.9fold, solubilized with 1% v/v Emulgen 909 | Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.49 | liver | - |
Gallus gallus | - |
| 3.1.4.49 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.49 | 0.00000011 | - |
pH 6.3 | Rattus norvegicus |
| 3.1.4.49 | 0.000631 | - |
pH 5.3, 37°C | Gallus gallus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.4.49 | 0°C, in the presence of 2-mercaptoethanol and stabilizing factor, 1 month, stable | Gallus gallus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.49 | mannosylphosphodolichol + H2O | - |
Rattus norvegicus | D-mannose + dolichyl phosphate | - |
? | |
| 3.1.4.49 | mannosylphosphodolichol + H2O | highly specific for Dol-P-Man | Gallus gallus | D-mannose + dolichyl phosphate | - |
? | |
| 3.1.4.49 | mannosylphosphodolichol + H2O | enzyme may be capable of degrading Dol-P-Man in vivo | Gallus gallus | D-mannose + dolichyl phosphate | - |
? | |
| 3.1.4.49 | additional information | not: N-acetylglucosaminyldiphosphodolichol, glucosylphosphodolichol or mannose 1-phosphate, artificial substrates for acid phosphatase, acid phosphodiesterase, mannosidase or glucosidase | Gallus gallus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.49 | 37 | - |
assay at | Gallus gallus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.49 | 5.3 | - |
acetate buffer | Gallus gallus |
| 3.1.4.49 | 6.3 | - |
assay at | Rattus norvegicus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.49 | 0.0125 | - |
dolichyl phosphate | pH 5.3, 37°C | Gallus gallus | |
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