BRENDA - Enzyme Database

Characterization and partial purification of a novel mannosylphosphodolichol phosphodiesterase from chicken liver microsomes

Tomita, Y.; Motokawa, Y.; Eur. J. Biochem. 170, 363-368 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.49
2-mercaptoethanol
requirement, or other SH-reducing agents
Gallus gallus
3.1.4.49
Emulgen 909
requirement, optimum concentration: 0.4% v/v
Gallus gallus
3.1.4.49
additional information
no requirement for any phospholipid
Gallus gallus
3.1.4.49
SH-reducing agents
e.g. 2-mercaptoethanol, absolute requirement
Gallus gallus
General Stability
EC Number
General Stability
Organism
3.1.4.49
a heat-stable factor, precipitable by trichloroacetic acid, insoluble in lipid solvents, stabilizes, separation from this factor by ion-exchange chromatography inactivates irreversibly
Gallus gallus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.49
bis(4-nitrophenyl)phosphate
1 mM, competitive inhibition
Gallus gallus
3.1.4.49
dolichol
0.1 mM, strong, competitive inhibition
Gallus gallus
3.1.4.49
dolichyl phosphate
0.1 mM, strong, competitive inhibition
Gallus gallus
3.1.4.49
additional information
not inhibited by D-mannose, mannose 1-phosphate, GDP, GMP, ADP, AMP, phosphate, diphosphate GDPmannose, UDP-N-acetylglucosamine, UDPglucose, UDPglucuronic acid, 4-nitrophenyl phosphate, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside or 4-nitrophenyl-beta-D-glucopyranoside
Gallus gallus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.49
0.00043
-
Mannosylphosphodolichol
pH 5.3, 37C
Gallus gallus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.49
microsome
predominantly
Gallus gallus
-
-
3.1.4.49
microsome
-
Rattus norvegicus
-
-
3.1.4.49
mitochondrion
-
Gallus gallus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.49
CaCl2
greatly stimulates, optimum concentration: 2 mM
Gallus gallus
3.1.4.49
additional information
not activated by other divalent cations than Ca2+
Gallus gallus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.49
mannosylphosphodolichol + H2O
Gallus gallus
enzyme may be capable of degrading Dol-P-Man in vivo
D-mannose + dolichyl phosphate
-
Gallus gallus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.49
Gallus gallus
-
-
-
3.1.4.49
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.49
partial, 57.9fold, solubilized with 1% v/v Emulgen 909
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.49
liver
-
Gallus gallus
-
3.1.4.49
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.4.49
0.00000011
-
pH 6.3
Rattus norvegicus
3.1.4.49
0.000631
-
pH 5.3, 37C
Gallus gallus
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.49
0C, in the presence of 2-mercaptoethanol and stabilizing factor, 1 month, stable
Gallus gallus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.49
mannosylphosphodolichol + H2O
-
646471
Rattus norvegicus
D-mannose + dolichyl phosphate
-
646471
Rattus norvegicus
?
3.1.4.49
mannosylphosphodolichol + H2O
highly specific for Dol-P-Man
646471
Gallus gallus
D-mannose + dolichyl phosphate
-
646471
Gallus gallus
?
3.1.4.49
mannosylphosphodolichol + H2O
enzyme may be capable of degrading Dol-P-Man in vivo
646471
Gallus gallus
D-mannose + dolichyl phosphate
-
646471
Gallus gallus
?
3.1.4.49
additional information
not: N-acetylglucosaminyldiphosphodolichol, glucosylphosphodolichol or mannose 1-phosphate, artificial substrates for acid phosphatase, acid phosphodiesterase, mannosidase or glucosidase
646471
Gallus gallus
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.49
37
-
assay at
Gallus gallus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.49
5.3
-
acetate buffer
Gallus gallus
3.1.4.49
6.3
-
assay at
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.4.49
0.0125
-
dolichyl phosphate
pH 5.3, 37C
Gallus gallus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.49
2-mercaptoethanol
requirement, or other SH-reducing agents
Gallus gallus
3.1.4.49
Emulgen 909
requirement, optimum concentration: 0.4% v/v
Gallus gallus
3.1.4.49
additional information
no requirement for any phospholipid
Gallus gallus
3.1.4.49
SH-reducing agents
e.g. 2-mercaptoethanol, absolute requirement
Gallus gallus
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.49
a heat-stable factor, precipitable by trichloroacetic acid, insoluble in lipid solvents, stabilizes, separation from this factor by ion-exchange chromatography inactivates irreversibly
Gallus gallus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.49
bis(4-nitrophenyl)phosphate
1 mM, competitive inhibition
Gallus gallus
3.1.4.49
dolichol
0.1 mM, strong, competitive inhibition
Gallus gallus
3.1.4.49
dolichyl phosphate
0.1 mM, strong, competitive inhibition
Gallus gallus
3.1.4.49
additional information
not inhibited by D-mannose, mannose 1-phosphate, GDP, GMP, ADP, AMP, phosphate, diphosphate GDPmannose, UDP-N-acetylglucosamine, UDPglucose, UDPglucuronic acid, 4-nitrophenyl phosphate, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside or 4-nitrophenyl-beta-D-glucopyranoside
Gallus gallus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.4.49
0.0125
-
dolichyl phosphate
pH 5.3, 37C
Gallus gallus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.49
0.00043
-
Mannosylphosphodolichol
pH 5.3, 37C
Gallus gallus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.49
microsome
predominantly
Gallus gallus
-
-
3.1.4.49
microsome
-
Rattus norvegicus
-
-
3.1.4.49
mitochondrion
-
Gallus gallus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.49
CaCl2
greatly stimulates, optimum concentration: 2 mM
Gallus gallus
3.1.4.49
additional information
not activated by other divalent cations than Ca2+
Gallus gallus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.49
mannosylphosphodolichol + H2O
Gallus gallus
enzyme may be capable of degrading Dol-P-Man in vivo
D-mannose + dolichyl phosphate
-
Gallus gallus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.49
partial, 57.9fold, solubilized with 1% v/v Emulgen 909
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.49
liver
-
Gallus gallus
-
3.1.4.49
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.4.49
0.00000011
-
pH 6.3
Rattus norvegicus
3.1.4.49
0.000631
-
pH 5.3, 37C
Gallus gallus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.49
0C, in the presence of 2-mercaptoethanol and stabilizing factor, 1 month, stable
Gallus gallus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.49
mannosylphosphodolichol + H2O
-
646471
Rattus norvegicus
D-mannose + dolichyl phosphate
-
646471
Rattus norvegicus
?
3.1.4.49
mannosylphosphodolichol + H2O
highly specific for Dol-P-Man
646471
Gallus gallus
D-mannose + dolichyl phosphate
-
646471
Gallus gallus
?
3.1.4.49
mannosylphosphodolichol + H2O
enzyme may be capable of degrading Dol-P-Man in vivo
646471
Gallus gallus
D-mannose + dolichyl phosphate
-
646471
Gallus gallus
?
3.1.4.49
additional information
not: N-acetylglucosaminyldiphosphodolichol, glucosylphosphodolichol or mannose 1-phosphate, artificial substrates for acid phosphatase, acid phosphodiesterase, mannosidase or glucosidase
646471
Gallus gallus
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.49
37
-
assay at
Gallus gallus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.49
5.3
-
acetate buffer
Gallus gallus
3.1.4.49
6.3
-
assay at
Rattus norvegicus