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Literature summary extracted from

  • Salvucci, M.E.; Holbrook, G.P.
    Purification and properties of 2-carboxy-D-arabinitol 1-phosphatase (1989), Plant Physiol., 90, 679-685.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.63 3-phosphoglycerate activation Nicotiana tabacum
3.1.3.63 D-fructose 1,6-bisphosphate activation Nicotiana tabacum
3.1.3.63 additional information no activation by ATP Nicotiana tabacum
3.1.3.63 additional information no activation by ADP, 6-phosphogluconate, D-fructose 6-phosphate, D-glucose 1-phosphate, D-ribulose 5-phosphate, D-ribose 5-phosphate Nicotiana tabacum
3.1.3.63 Ribulose 1,5-bisphosphate activation Nicotiana tabacum

General Stability

EC Number General Stability Organism
3.1.3.63 major loss of activity during ion-exchange chromatography Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.63 ATP
-
 Nicotiana tabacum
3.1.3.63 Cu2+ 5 mM Nicotiana tabacum
3.1.3.63 MoO42- strong Nicotiana tabacum
3.1.3.63 additional information no inhibition by K+, Mg2+, Na+, NH4+, EDTA or cysteine Nicotiana tabacum
3.1.3.63 PO43-
-
 Nicotiana tabacum
3.1.3.63 ribose 5-phosphate weak Nicotiana tabacum
3.1.3.63 SO42- inhibitory above 700 mM, activates at 10-50 mM Nicotiana tabacum
3.1.3.63 Zn2+ 5 mM Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.63 0.033
-
 2-carboxyarabinitol 1-phosphate 25°C, pH 7.5 Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.63 chloroplast
-
 Nicotiana tabacum 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.63 additional information no activation by Mg2+ Nicotiana tabacum
3.1.3.63 additional information no activation by K+, Na+, NH4+ Nicotiana tabacum
3.1.3.63 SO42- activation, 10-50 mM, inhibits above 700 mM Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.63 53000
-
 x * 53000, SDS-PAGE Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.63 2-carboxy-D-arabinitol 1-phosphate + H2O Nicotiana tabacum
-
 2-carboxy-D-arabinitol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.63 Nicotiana tabacum
-
 tobacco, var. KY14
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.63 from leaf and from isolated chloroplasts Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.63 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.63 0.2612
-
 25°C, pH 7.5 Nicotiana tabacum

Storage Stability

EC Number Storage Stability Organism
3.1.3.63 -20°C, slow loss of activity in the absence of ammonium sulfate Nicotiana tabacum
3.1.3.63 4°C, stable as ammonium sulfate suspension, slow loss of activity in the absence of ammonium sulfate Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.63 2-carboxy-D-arabinitol 1-phosphate + H2O
-
 Nicotiana tabacum 2-carboxy-D-arabinitol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.63 ? x * 53000, SDS-PAGE Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.63 25
-
 assay at Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.63 7.5
-
-
 Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.63 5.5 8.4 about 65% of maximal activity at pH 5.5 and about half-maximal activity at pH 8.4 Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.3.63 NADPH activation Nicotiana tabacum