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Literature summary extracted from

  • Communi, D.; Lecocq, R.; Erneux, C.
    Arginine 343 and 350 are two active site residues involved in substrate binding by human type I D-myo-inositol 1,4,5-trisphosphate 5-phosphatase (1996), J. Biol. Chem., 271, 11676-11683.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.56 expressed in HB 101 host bacteria Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.56 R343A increased Km and dramatic loss of activity Homo sapiens
3.1.3.56 R350A increased Km Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.56 2,3-bisphosphoglycerate
-
 Homo sapiens
3.1.3.56 Phenylglyoxal Arg-343 and Arg-350 were found to be covalently modified; irreversible, substrates and 2,3-bisphosphoglycerate protect against inactivation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.56 0.021
-
 D-myo-inositol 1,4,5-trisphosphate native enzyme Homo sapiens
3.1.3.56 0.205
-
 D-myo-inositol 1,4,5-trisphosphate R343A mutant Homo sapiens
3.1.3.56 0.32
-
 D-myo-inositol 1,4,5-trisphosphate R350A mutant Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.56 43000
-
 SDS-PAGE, type I enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O Homo sapiens regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.56 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.56 recombinant enzyme using His-tag Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.56 31
-
 Vmax, R343A mutant Homo sapiens
3.1.3.56 191
-
 Vmax, native enzyme Homo sapiens
3.1.3.56 237
-
 Vmax, R350A mutant Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Homo sapiens D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Homo sapiens D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?