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Literature summary extracted from

  • Hansen, C.A.; Johanson, R.A.; Williamson, M.T.; Williamson, J.R.
    Purification and characterization of two types of soluble inositol phosphate 5-phosphomonoesterases from rat brain (1987), J. Biol. Chem., 262, 17319-17326.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.56 2,3-bisphosphoglycerate
-
 Rattus norvegicus
3.1.3.56 ADP
-
 Rattus norvegicus
3.1.3.56 AMP slight inhibition Rattus norvegicus
3.1.3.56 ATP
-
 Rattus norvegicus
3.1.3.56 Ca2+ above 0.001 mM Rattus norvegicus
3.1.3.56 D-fructose 1,6-bisphosphate
-
 Rattus norvegicus
3.1.3.56 D-fructose 2,6-bisphosphate
-
 Rattus norvegicus
3.1.3.56 D-glucose 6-phosphate slight inhibition Rattus norvegicus
3.1.3.56 EDTA no activity in presence of 1 mM EDTA Rattus norvegicus
3.1.3.56 Mg2+ above 0.5 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.56 0.0008
-
 D-myo-Inositol 1,3,4,5-tetrakisphosphate type I enzyme, pH 7.1, 30°C Rattus norvegicus
3.1.3.56 0.003
-
 D-myo-inositol 1,4,5-trisphosphate type I enzyme, pH 7.1, 30°C Rattus norvegicus
3.1.3.56 0.018
-
 D-myo-inositol 1,4,5-trisphosphate type II enzyme, pH 7.1, 30°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.56 soluble two soluble forms, together both forms represent 26% of total activity Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.56 Ca2+ moderate inhibition Rattus norvegicus
3.1.3.56 Mg2+ required, maximum activity with 0.01 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.56 60000
-
 gel filtration and SDS-PAGE, type I enzyme Rattus norvegicus
3.1.3.56 160000
-
 gel filtration and SDS-PAGE, type II enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.56 D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O Rattus norvegicus
-
 D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O Rattus norvegicus regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.56 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.56
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.56 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.56 1.06
-
 purified type I enzyme, pH 7.1, 30°C Rattus norvegicus
3.1.3.56 15.2
-
 purified type II enzyme, pH 7.1, 30°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.3.56 -20°C, type II stable, type I lost 10% of activity per week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.56 D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Rattus norvegicus D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O substrate for type I but not type II enzyme Rattus norvegicus D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O at 8% of the rate of inositol 1,4,5-trisphosphate hydrolysis Rattus norvegicus D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Rattus norvegicus D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.56 monomer 1 * 60000, gel filtration, SDS-PAGE, type I, 1 * 160000, gel filtration, SDS-PAGE, type II Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.56 6.5 7 type II enzyme Rattus norvegicus
3.1.3.56 7.5
-
 type I enzyme Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.56 6 9
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.56 0.07
-
 Ca2+
-
 Rattus norvegicus