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Literature summary extracted from

  • Peebles, C.L.; Gegenheimer, P.; Abelson, J.
    Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease (1983), Cell, 32, 525-536.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.16 nonionic detergents stimulate activity of the endonuclease in the membrane fraction Saccharomyces cerevisiae
4.6.1.16 spermidine or spermine required Saccharomyces cerevisiae
4.6.1.16 spermine or spermidine required, enhances extent and accuracy of cleavage, degree of stimulation varies with the pre-tRNA substrate Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.16 Co2+
-
 Saccharomyces cerevisiae
4.6.1.16 Fe2+
-
 Saccharomyces cerevisiae
4.6.1.16 Mg2+ at high concentrations Saccharomyces cerevisiae
4.6.1.16 Mn2+
-
 Saccharomyces cerevisiae
4.6.1.16 monovalent cations at high concentrations Saccharomyces cerevisiae
4.6.1.16 Ni2+
-
 Saccharomyces cerevisiae
4.6.1.16 p-hydroxymercuribenzoate
-
 Saccharomyces cerevisiae
4.6.1.16 Zn2+
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.16 membrane integral membrane protein Saccharomyces cerevisiae 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.16 tRNA precursor Saccharomyces cerevisiae
-
 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor Saccharomyces cerevisiae M304
-
 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.16 Saccharomyces cerevisiae
-
-
-
4.6.1.16 Saccharomyces cerevisiae M304
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.16 partial, strain M304 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.16 tRNA precursor
-
 Saccharomyces cerevisiae a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini Saccharomyces cerevisiae a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor specifically removing the intron Saccharomyces cerevisiae a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor
-
 Saccharomyces cerevisiae M304 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini Saccharomyces cerevisiae M304 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor specifically removing the intron Saccharomyces cerevisiae M304 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.16 30
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.16 37
-
 rapid inactivation above Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.6.1.16 7 8 active in the range Saccharomyces cerevisiae