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Literature summary extracted from

  • Hwu, L.; Huang, K.; Chen, D.; Lin, A.
    The action mode of the ribosome-inactivating protein alpha-sarcin (2000), J. Biomed. Sci., 7, 420-428.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.23 cloning of the genomic alpha-sarcin gene pGEM-T/FL-alpha plasmid, construction of the alpha-sarcin gene and its mutants by PCR, using genomic DNA as template Aspergillus giganteus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.23 C148S point mutation constructed from cDNA clone Aspergillus giganteus
4.6.1.23 C6S point mutation constructed from cDNA clone Aspergillus giganteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.23 rRNA + H2O Aspergillus giganteus
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.23 Aspergillus giganteus
-
filamentous fungus, mold
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.23 recombinant enzyme Aspergillus giganteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.23 RNA + H2O hydrolyzes naked RNA at purines in both single-and double-stranded regions Aspergillus giganteus ?
-
?
4.6.1.23 rRNA + H2O
-
Aspergillus giganteus ?
-
?