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Literature summary extracted from

  • Tilton, G.; Shockey, J.; Browse, J.
    Two families of acyl-CoA thioesterases in Arabidopsis (2000), Biochem. Soc. Trans., 28, 946-947.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.2.2 biotechnology enzyme is involved in fatty acid biosynthesis and may be a good target for improvement of special oil production in transgenic plants Arabidopsis thaliana
3.1.2.2 biotechnology enzyme can be used for bioengineering of transgenic plants to produce oils with desired properties Arabidopsis thaliana
3.1.2.20 biotechnology enzyme is involved in fatty acid biosynthesis and may be a good target for improvement of special oil production in transgenic plants Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 functional overexpression of AtACH2 in Escherichia coli, overexpression of AtACH5 fused to the green fluorescent protein in Escherichia coli Arabidopsis thaliana
3.1.2.2 overexpression of isozymes AtACH5and AtACH2 in Escherichia coli , AtACH2 fused to maltose-binding protein Arabidopsis thaliana
3.1.2.20 overexpression of isozymes AtACH5and AtACH2 in Escherichia coli , AtACH2 fused to maltose-binding protein Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.2 additional information a AtACH5 gene knock-out mutant shows no altered phenotype Arabidopsis thaliana
3.1.2.2 additional information AtACH5 knockout mutants do not have an altered phenotype Arabidopsis thaliana
3.1.2.20 additional information a AtACH5 gene knock-out mutant shows no altered phenotype Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 endoplasmic reticulum isozymes AtACH4 and AtACH5 Arabidopsis thaliana 5783
-
3.1.2.2 endoplasmic reticulum isozyme family built of ACH4 and ACH5 Arabidopsis thaliana 5783
-
3.1.2.2 peroxisome isozymes AtACH1 and AtACH2 Arabidopsis thaliana 5777
-
3.1.2.2 peroxisome isozyme family built of ACH1 and ACH2 Arabidopsis thaliana 5777
-
3.1.2.20 endoplasmic reticulum isozymes AtACH4 and AtACH5 Arabidopsis thaliana 5783
-
3.1.2.20 peroxisome isozymes AtACH1 and AtACH2 Arabidopsis thaliana 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 acyl-CoA + H2O Arabidopsis thaliana
-
 CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O Arabidopsis thaliana
-
 CoA + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Arabidopsis thaliana
-
 2 families of acyl-CoA thioesterases due to their localization: 1. isozymes ACH1 and ACH2, 2. isozymes ACH4 and ACH5
-
3.1.2.2 Arabidopsis thaliana
-
 2 families of acyl-CoA thioesterases: 1. peroxisomal family comprising ACH1 and ACH2, 2. endoplasmic family comprising ACH4 and ACH5
-
3.1.2.20 Arabidopsis thaliana
-
 2 families of acyl-CoA thioesterases due to their localization: 1. isozymes ACH1 and ACH2, 2. isozymes ACH4 and ACH5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 partial purification of recombinant ACH2 overexpressed in Escherichia coli Arabidopsis thaliana
3.1.2.2 partially, recombinant isozyme AtACH2 fused to maltose-binding protein from Escherichia coli Arabidopsis thaliana
3.1.2.20 partially, recombinant isozyme AtACH2 fused to maltose-binding protein from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O
-
 Arabidopsis thaliana CoA + a carboxylate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O
-
 Arabidopsis thaliana CoA + hexadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Arabidopsis thaliana CoA + oleate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Arabidopsis thaliana CoA + palmitate
-
 ?
3.1.2.20 acyl-CoA + H2O
-
 Arabidopsis thaliana CoA + a carboxylate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O
-
 Arabidopsis thaliana CoA + hexadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Arabidopsis thaliana CoA + oleate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.2 ACH
-
 Arabidopsis thaliana
3.1.2.2 acyl-CoA thioesterase
-
 Arabidopsis thaliana