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Literature summary extracted from

  • Tilton, G.B.; Shockey, J.M.; Browse, J.
    Biochemical and molecular characterization of ACH2, an acyl-CoA thioesterase from arabidopsis thaliana (2004), J. Biol. Chem., 279, 7487-7494.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 DNA sequence determination and analysis of isolated and commercial clone, overexpression of His-tagged isozyme ACH2 in Escherichia coli BL21(DE3) cells Arabidopsis thaliana
3.1.2.2 gene ACH2, DNA and amino acid sequence determination and analysis, overexpression as C-terminally His-tagged protein in Escherichia coli BL21(DE3) Arabidopsis thaliana
3.1.2.2 overexpression in Escherichia coli BL-21(DE3) as a His-tagged protein Arabidopsis thaliana
3.1.2.20 DNA sequence determination and analysis of isolated and commercial clone, overexpression of His-tagged isozyme ACH2 in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 acyl-CoA e.g. lauroyl-CoA, at 0.005 mM, can be overcome by addition of bovine serum albumin or alpha-casein Arabidopsis thaliana
3.1.2.2 CoASH feedback inhibition of recombinant enzyme at concentrations above 0.1 mM Arabidopsis thaliana
3.1.2.2 DTNB above 0.3 mM; above 0.3 mM Arabidopsis thaliana
3.1.2.2 additional information reversible substrate inhibition by long-chain acyl-CoAs at concentration 0.005 mM, reversibel by bovine serum albumin and alpha-casein Arabidopsis thaliana
3.1.2.20 acyl-CoA e.g. lauroyl-CoA, at 0.005 mM, can be overcome by addition of bovine serum albumin or alpha-casein Arabidopsis thaliana
3.1.2.20 CoASH feedback inhibition of recombinant enzyme at concentrations above 0.1 mM Arabidopsis thaliana
3.1.2.20 DTNB above 0.3 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 additional information
-
additional information kinetics Arabidopsis thaliana
3.1.2.2 0.0058
-
oleoyl-CoA
-
Arabidopsis thaliana
3.1.2.2 0.0058
-
palmitoleoyl-CoA pH 8.0 Arabidopsis thaliana
3.1.2.2 0.0178
-
dodecanoyl-CoA
-
Arabidopsis thaliana
3.1.2.2 0.018
-
dodecanoyl-CoA pH 8.0 Arabidopsis thaliana
3.1.2.20 additional information
-
additional information kinetics Arabidopsis thaliana
3.1.2.20 0.0058
-
palmitoleoyl-CoA pH 8.0 Arabidopsis thaliana
3.1.2.20 0.018
-
dodecanoyl-CoA pH 8.0 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 peroxisome isozyme ACH2 Arabidopsis thaliana 5777
-
3.1.2.20 peroxisome isozyme ACH2 Arabidopsis thaliana 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 196000
-
recombinant isozyme ACH2, analytical ultracentrifugation Arabidopsis thaliana
3.1.2.20 196000
-
recombinant isozyme ACH2, analytical ultracentrifugation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 acyl-CoA + H2O Arabidopsis thaliana enzyme is not linked to fatty acid oxidation CoA + a carboxylate
-
?
3.1.2.2 palmitoleoyl-CoA + H2O Arabidopsis thaliana activity of ACH2 is not linked to fatty acid oxidation CoA + palmitoleate
-
?
3.1.2.20 acyl-CoA + H2O Arabidopsis thaliana enzyme is not linked to fatty acid oxidation CoA + a carboxylate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Arabidopsis thaliana
-
isozyme ACH2
-
3.1.2.2 Arabidopsis thaliana
-
Wassilewskija ecotype
-
3.1.2.2 Arabidopsis thaliana Q8GYW7
-
-
3.1.2.2 Arabidopsis thaliana Q8GYW7 isozyme ACH2
-
3.1.2.20 Arabidopsis thaliana
-
isozyme ACH2
-
3.1.2.20 Arabidopsis thaliana
-
Wassilewskija ecotype
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 recombinant Arabidopsis thaliana
3.1.2.2 recombinant His-tagged isozyme ACH2 from Escherichia coli Arabidopsis thaliana
3.1.2.20 recombinant His-tagged isozyme ACH2 from Escherichia coli Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.2 palmitoyl-CoA + H2O = CoA + palmitate catalytic triad D337-Q409-S359 Arabidopsis thaliana
3.1.2.20 an acyl-CoA + H2O = CoA + a carboxylate catalytic triad D337-Q409-S359 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 flower
-
Arabidopsis thaliana
-
3.1.2.2 leaf young Arabidopsis thaliana
-
3.1.2.2 additional information expression level during plant development Arabidopsis thaliana
-
3.1.2.2 seed
-
Arabidopsis thaliana
-
3.1.2.2 seedling
-
Arabidopsis thaliana
-
3.1.2.20 flower
-
Arabidopsis thaliana
-
3.1.2.20 leaf young Arabidopsis thaliana
-
3.1.2.20 seedling
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 21
-
purified recombinant enzyme, substrate palmitoleoyl-CoA Arabidopsis thaliana
3.1.2.2 21
-
activity with palmitoleoyl-CoA Arabidopsis thaliana
3.1.2.20 21
-
purified recombinant enzyme, substrate palmitoleoyl-CoA Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 4°C, pH 6.0, activity of recombinant His-tagged enzyme remains constant for 3 days Arabidopsis thaliana
3.1.2.2 4°C, pH 6.0, fully stable for 3 days, 90% remaining activity at pH 8.0 after 3 days Arabidopsis thaliana
3.1.2.2 4°C, pH 8.0, 3 days, recombinant His-tagged enzyme retains 90% of the original activity Arabidopsis thaliana
3.1.2.2 4°C, purified recombinant HIs-tagged enzyme, pH 6.0, no loss of activity after 3 days Arabidopsis thaliana
3.1.2.2 4°C, purified recombinant HIs-tagged enzyme, pH 8.0, 90% remaining activity after 3 days Arabidopsis thaliana
3.1.2.20 4°C, purified recombinant HIs-tagged enzyme, pH 6.0, no loss of activity after 3 days Arabidopsis thaliana
3.1.2.20 4°C, purified recombinant HIs-tagged enzyme, pH 8.0, 90% remaining activity after 3 days Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O prefers unsaturated acyl-CoA Arabidopsis thaliana CoA + a carboxylate
-
?
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Arabidopsis thaliana CoA + a carboxylate
-
?
3.1.2.2 acyl-CoA + H2O enzyme is not linked to fatty acid oxidation Arabidopsis thaliana CoA + a carboxylate
-
?
3.1.2.2 arachidonoyl-CoA + H2O
-
Arabidopsis thaliana CoA + arachidonate
-
?
3.1.2.2 dodecanoyl-CoA + H2O
-
Arabidopsis thaliana CoA + dodecanoate
-
?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Arabidopsis thaliana CoA + dodecanoate
-
?
3.1.2.2 lauroyl-CoA + H2O
-
Arabidopsis thaliana CoA + laurate
-
?
3.1.2.2 linoleoyl-CoA + H2O
-
Arabidopsis thaliana CoA + linoleoate
-
?
3.1.2.2 additional information broad substrate specificity, preference for long-chain unsaturated fatty acids Arabidopsis thaliana ?
-
?
3.1.2.2 myristoyl-CoA + H2O
-
Arabidopsis thaliana CoA + myristate
-
?
3.1.2.2 oleoyl-CoA + H2O
-
Arabidopsis thaliana CoA + oleate
-
?
3.1.2.2 palmitoleoyl-CoA
-
Arabidopsis thaliana CoA + CoA + palmitoleoate
-
?
3.1.2.2 palmitoleoyl-CoA + H2O
-
Arabidopsis thaliana CoA + palmitoleoate
-
?
3.1.2.2 palmitoleoyl-CoA + H2O best substrate Arabidopsis thaliana CoA + palmitoleoate
-
?
3.1.2.2 palmitoleoyl-CoA + H2O
-
Arabidopsis thaliana CoA + palmitoleate
-
?
3.1.2.2 palmitoleoyl-CoA + H2O activity of ACH2 is not linked to fatty acid oxidation Arabidopsis thaliana CoA + palmitoleate
-
?
3.1.2.2 palmitoyl-CoA + H2O
-
Arabidopsis thaliana CoA + palmitate
-
?
3.1.2.2 stearoyl-CoA + H2O
-
Arabidopsis thaliana CoA + stearate
-
?
3.1.2.20 acyl-CoA + H2O prefers unsaturated acyl-CoA Arabidopsis thaliana CoA + a carboxylate
-
?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Arabidopsis thaliana CoA + a carboxylate
-
?
3.1.2.20 acyl-CoA + H2O enzyme is not linked to fatty acid oxidation Arabidopsis thaliana CoA + a carboxylate
-
?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Arabidopsis thaliana CoA + dodecanoate
-
?
3.1.2.20 palmitoleoyl-CoA + H2O best substrate Arabidopsis thaliana CoA + palmitoleoate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 tetramer
-
Arabidopsis thaliana
3.1.2.20 tetramer
-
Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.2.2 ACH2
-
Arabidopsis thaliana
3.1.2.2 acyl-CoA hydrolase
-
Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8 9
-
Arabidopsis thaliana
3.1.2.20 8 9
-
Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 7.5 9.5 pH 7.5: about 40% of maximal activity, pH 9.5: about 40% of maximal activity Arabidopsis thaliana