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Literature summary extracted from

  • Cheesbrough, T.M.; Kolattukudy, P.E.
    Purification and characterization of a fatty acyl-CoA hydrolase from the uropygial glands of Peking ducks (Anas domesticus) (1985), Arch. Biochem. Biophys., 237, 208-216.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.2.2 no dithioerythritol or other mercaptam required to maintain activity Anas platyrhynchos
3.1.2.20 no dithioerythritol or other mercaptam required to maintain activity Anas platyrhynchos

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 23% inhibition at 10 mM Anas platyrhynchos
3.1.2.2 diethyldicarbonate 95% inhibition at 2 mM Anas platyrhynchos
3.1.2.2 diisopropylfluorophosphate 94% inhibition at 5 mM, maximal inhibition Anas platyrhynchos
3.1.2.2 p-hydroxymercuribenzoate 99% inhibition at 0.1 mM Anas platyrhynchos
3.1.2.2 PMSF 96% inhibition at 1 mM Anas platyrhynchos
3.1.2.20 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 23% inhibition at 10 mM Anas platyrhynchos
3.1.2.20 diethyldicarbonate 95% inhibition at 2 mM Anas platyrhynchos
3.1.2.20 diisopropylfluorophosphate 94% inhibition at 5 mM, maximal inhibition Anas platyrhynchos
3.1.2.20 p-hydroxymercuribenzoate 99% inhibition at 0.1 mM Anas platyrhynchos
3.1.2.20 PMSF 96% inhibition at 1 mM Anas platyrhynchos

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
 Anas platyrhynchos 5829
-
3.1.2.20 cytosol
-
 Anas platyrhynchos 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 56300
-
 gel filtration Anas platyrhynchos
3.1.2.20 56300
-
 gel filtration Anas platyrhynchos

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 acyl-CoA + H2O Anas platyrhynchos
-
 CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O Anas platyrhynchos
-
 CoA + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Anas platyrhynchos
-
 4 isoforms of different pI and MW
-
3.1.2.2 Anas platyrhynchos
-
 Peking or mallard duck
-
3.1.2.20 Anas platyrhynchos
-
 4 isoforms of different pI and MW
-
3.1.2.20 Anas platyrhynchos
-
 Peking or mallard duck
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 184fold Anas platyrhynchos
3.1.2.20 184fold Anas platyrhynchos

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 uropygial gland
-
 Anas platyrhynchos
-
3.1.2.20 uropygial gland
-
 Anas platyrhynchos
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 45.4
-
 partially purified enzyme Anas platyrhynchos
3.1.2.20 45.4
-
 partially purified enzyme Anas platyrhynchos

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -20°C, purified lyophilized, at least 6 months Anas platyrhynchos
3.1.2.20 -20°C, purified lyophilized, at least 6 months Anas platyrhynchos

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O
-
 Anas platyrhynchos CoA + a carboxylate
-
 ?
3.1.2.2 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Anas platyrhynchos CoA + a carboxylate
-
 ?
3.1.2.2 decanoyl-CoA + H2O
-
 Anas platyrhynchos CoA + decanoate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Anas platyrhynchos CoA + dodecanoate
-
 ?
3.1.2.2 additional information substrate specificity alters with pH Anas platyrhynchos ?
-
 ?
3.1.2.2 octanoyl-CoA + H2O
-
 Anas platyrhynchos CoA + octanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Anas platyrhynchos CoA + tetradecanoate
-
 ?
3.1.2.20 acyl-CoA + H2O
-
 Anas platyrhynchos CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Anas platyrhynchos CoA + a carboxylate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Anas platyrhynchos CoA + decanoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Anas platyrhynchos CoA + dodecanoate
-
 ?
3.1.2.20 additional information substrate specificity alters with pH Anas platyrhynchos ?
-
 ?
3.1.2.20 octanoyl-CoA + H2O
-
 Anas platyrhynchos CoA + octanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Anas platyrhynchos CoA + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 monomer 1 * 55800-59400, 4 isoforms, SDS-PAGE Anas platyrhynchos
3.1.2.20 monomer 1 * 55800-59400, 4 isoforms, SDS-PAGE Anas platyrhynchos

Synonyms

EC Number Synonyms Comment Organism
3.1.2.2 More cf. EC 3.1.2.19 Anas platyrhynchos
3.1.2.2 More immunologically not related with the thioesterase of fatty acid synthase or the acyl fatty acid synthase thioester hydrolase Anas platyrhynchos
3.1.2.20 More cf. EC 3.1.2.19 Anas platyrhynchos
3.1.2.20 More immunologically not related with the thioesterase of fatty acid synthase or the acyl fatty acid synthase thioester hydrolase Anas platyrhynchos

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 30
-
 assay at Anas platyrhynchos
3.1.2.20 30
-
 assay at Anas platyrhynchos

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 additional information
-
-
 Anas platyrhynchos
3.1.2.2 9.3
-
 dodecanoyl-CoA as substrate Anas platyrhynchos
3.1.2.20 additional information
-
-
 Anas platyrhynchos
3.1.2.20 9.3
-
 dodecanoyl-CoA as substrate Anas platyrhynchos

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 7.6 10 about half-maximal activity at pH 7.6 and pH 10.0, dodecanoyl-CoA as substrate Anas platyrhynchos
3.1.2.20 7.6 10 about half-maximal activity at pH 7.6 and pH 10.0, dodecanoyl-CoA as substrate Anas platyrhynchos

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.2.2 Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.4
3.1.2.2 Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.45
3.1.2.2 Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes 4.8 4.7
3.1.2.2 Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.9
3.1.2.20 Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.4
3.1.2.20 Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.45
3.1.2.20 Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes 4.8 4.7
3.1.2.20 Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.9