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Literature summary extracted from

  • Seay, T.; Lucking, D.R.
    Purification and properties of acyl coenzyme A thioesterase II from Rhodopseudomonas sphaeroides (1986), Biochemistry, 25, 2480-2485.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.2.2 glycerol or ethylene glycol stabilize Cereibacter sphaeroides
3.1.2.20 glycerol or ethylene glycol stabilize Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 diisopropylfluorophosphate no inhibition Cereibacter sphaeroides
3.1.2.20 diisopropylfluorophosphate no inhibition Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.0004
-
 octadecanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.2 0.0005
-
 hexadecanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.2 0.0017
-
 tetradecanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.2 0.0018
-
 dodecanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.2 0.0027
-
 decanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.2 0.0055
-
 hexanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.2 0.007
-
 octanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.2 0.013
-
 butyryl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.20 0.0004
-
 octadecanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.20 0.0005
-
 hexadecanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.20 0.0017
-
 tetradecanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.20 0.0018
-
 dodecanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.20 0.0027
-
 decanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.20 0.0055
-
 hexanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.20 0.007
-
 octanoyl-CoA pH 7.8, 22°C Cereibacter sphaeroides
3.1.2.20 0.013
-
 butyryl-CoA pH 7.8, 22°C Cereibacter sphaeroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
 Cereibacter sphaeroides 5829
-
3.1.2.20 cytosol
-
 Cereibacter sphaeroides 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 120000
-
 native PAGE Cereibacter sphaeroides
3.1.2.20 120000
-
 native PAGE Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 acyl-CoA + H2O Cereibacter sphaeroides
-
 CoA + a carboxylate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O Cereibacter sphaeroides preferred substrate CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O Cereibacter sphaeroides i.e. palmitoyl-CoA CoA + hexadecanoate
-
 ?
3.1.2.20 acyl-CoA + H2O Cereibacter sphaeroides
-
 CoA + a carboxylate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O Cereibacter sphaeroides preferred substrate CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O Cereibacter sphaeroides i.e. palmitoyl-CoA CoA + hexadecanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Cereibacter sphaeroides
-
-
-
3.1.2.20 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 5300fold Cereibacter sphaeroides
3.1.2.20 5300fold Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 41.6
-
 purified enzyme, decanoyl-CoA as substrate Cereibacter sphaeroides
3.1.2.20 41.6
-
 purified enzyme, decanoyl-CoA as substrate Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -20°C, in 20% v/v glycerol or ethylene glycol, t1/2: 1 year Cereibacter sphaeroides
3.1.2.2 4°C, in 50-70% ethylene glycol, t1/2: 60 days, upon removal of ethylene glycol: t1/2: 15 days Cereibacter sphaeroides
3.1.2.2 room temperature, not more than 1 day Cereibacter sphaeroides
3.1.2.20 -20°C, in 20% v/v glycerol or ethylene glycol, t1/2: 1 year Cereibacter sphaeroides
3.1.2.20 4°C, in 50-70% ethylene glycol, t1/2: 60 days, upon removal of ethylene glycol: t1/2: 15 days Cereibacter sphaeroides
3.1.2.20 room temperature, not more than 1 day Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 3-hydroxydecanoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + 3-hydroxydecanoate
-
 ?
3.1.2.2 acyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + a carboxylate
-
 ?
3.1.2.2 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Cereibacter sphaeroides CoA + a carboxylate
-
 ?
3.1.2.2 acyl-CoA + H2O saturated and unsaturated acyl-CoA Cereibacter sphaeroides CoA + a carboxylate
-
 ?
3.1.2.2 decanoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + decanoate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Cereibacter sphaeroides CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O best substrate Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O preferred substrate Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
3.1.2.2 hexanoyl-CoA + H2O poor substrate Cereibacter sphaeroides CoA + hexanoate
-
 ?
3.1.2.2 n-butyryl-CoA + H2O
-
 Cereibacter sphaeroides CoA + n-butanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O preferred substrate Cereibacter sphaeroides CoA + octadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Cereibacter sphaeroides CoA + octadecanoate
-
 ?
3.1.2.2 octanoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + octanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + tetradecanoate
-
 ?
3.1.2.2 vaccenoyl-CoA + H2O i.e. [trans]-11-octadecenoyl-CoA, preferred substrate of unsaturated acyl-CoA Cereibacter sphaeroides CoA + vaccenoate
-
 ?
3.1.2.20 3-hydroxydecanoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + 3-hydroxydecanoate
-
 ?
3.1.2.20 acyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Cereibacter sphaeroides CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O saturated and unsaturated acyl-CoA Cereibacter sphaeroides CoA + a carboxylate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + decanoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Cereibacter sphaeroides CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O best substrate Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O preferred substrate Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
3.1.2.20 hexanoyl-CoA + H2O poor substrate Cereibacter sphaeroides CoA + hexanoate
-
 ?
3.1.2.20 n-butyryl-CoA + H2O
-
 Cereibacter sphaeroides CoA + n-butanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O preferred substrate Cereibacter sphaeroides CoA + octadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Cereibacter sphaeroides CoA + octadecanoate
-
 ?
3.1.2.20 octanoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + octanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + tetradecanoate
-
 ?
3.1.2.20 vaccenoyl-CoA + H2O i.e. [trans]-11-octadecenoyl-CoA, preferred substrate of unsaturated acyl-CoA Cereibacter sphaeroides CoA + vaccenoate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 20
-
 assay at Cereibacter sphaeroides
3.1.2.20 20
-
 assay at Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8
-
 assay at Cereibacter sphaeroides
3.1.2.2 8.3 8.5
-
 Cereibacter sphaeroides
3.1.2.20 8
-
 assay at Cereibacter sphaeroides
3.1.2.20 8.3 8.5
-
 Cereibacter sphaeroides