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Literature summary extracted from

  • Engberg, S.T.; Aoyama, T.; Alexson, S.E.H.; Hashimoto, T.; Svensson, L.T.
    Peroxisome proliferator-induced acyl-CoA thioesterase from rat liver cytosol: molecular cloning and functional expression in chinese hamster ovary cells (1997), Biochem. J., 323, 525-531.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 Clofibrate stimulates Rattus norvegicus
3.1.2.2 di(2-ethylhexyl)phthalate
-
 Rattus norvegicus
3.1.2.20 Clofibrate stimulates Rattus norvegicus
3.1.2.20 di(2-ethylhexyl)phthalate
-
 Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 DNA sequence determination and analysis, subcloning in Escherichia coli, and expression in CHO cells Rattus norvegicus
3.1.2.2 expression of cDNA in chinese hamster ovary cells Rattus norvegicus
3.1.2.20 DNA sequence determination and analysis, subcloning in Escherichia coli, and expression in CHO cells Rattus norvegicus
3.1.2.20 expression of cDNA in chinese hamster ovary cells Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.002
-
 hexadecanoyl-CoA
-
 Cricetulus griseus
3.1.2.2 0.01
-
 hexadecanoyl-CoA recombinant enzyme Rattus norvegicus
3.1.2.20 0.002
-
 hexadecanoyl-CoA
-
 Cricetulus griseus
3.1.2.20 0.01
-
 hexadecanoyl-CoA recombinant enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
 Rattus norvegicus 5829
-
3.1.2.2 microsome
-
 Rattus norvegicus
-
-
3.1.2.20 cytosol
-
 Rattus norvegicus 5829
-
3.1.2.20 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 40000
-
 x * 40000, SDS-PAGE Rattus norvegicus
3.1.2.20 40000
-
 x * 40000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 hexadecanoyl-CoA + H2O Cricetulus griseus i.e. palmitoyl-CoA CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O Rattus norvegicus i.e. palmitoyl-CoA CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O Cricetulus griseus i.e. palmitoyl-CoA CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O Rattus norvegicus i.e. palmitoyl-CoA CoA + hexadecanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Cricetulus griseus
-
-
-
3.1.2.2 Rattus norvegicus
-
-
-
3.1.2.2 Rattus norvegicus Q64559 male sprague-dawley rats
-
3.1.2.20 Cricetulus griseus
-
-
-
3.1.2.20 Rattus norvegicus
-
-
-
3.1.2.20 Rattus norvegicus Q64559 male sprague-dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 native enzyme from liver Rattus norvegicus
3.1.2.20 native enzyme from liver Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 CHO cell
-
 Cricetulus griseus
-
3.1.2.2 liver
-
 Rattus norvegicus
-
3.1.2.20 CHO cell
-
 Cricetulus griseus
-
3.1.2.20 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 0.086
-
 CHO cells, substrate palmitoyl-CoA Cricetulus griseus
3.1.2.2 0.5
-
 recombinant enzyme in CHO cells, substrate palmitoyl-CoA Rattus norvegicus
3.1.2.2 250
-
-
 Rattus norvegicus
3.1.2.20 0.086
-
 CHO cells, substrate palmitoyl-CoA Cricetulus griseus
3.1.2.20 0.5
-
 recombinant enzyme in CHO cells, substrate palmitoyl-CoA Rattus norvegicus
3.1.2.20 250
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O
-
 Cricetulus griseus CoA + a carboxylate
-
 ?
3.1.2.2 acyl-CoA + H2O
-
 Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.2 decanoyl-CoA + H2O
-
 Cricetulus griseus CoA + decanoate
-
 ?
3.1.2.2 decanoyl-CoA + H2O
-
 Rattus norvegicus CoA + decanoate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O
-
 Cricetulus griseus CoA + dodecanoate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O
-
 Rattus norvegicus CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Cricetulus griseus CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O
-
 Cricetulus griseus CoA + octadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O
-
 Rattus norvegicus CoA + octadecanoate
-
 ?
3.1.2.2 octanoyl-CoA + H2O
-
 Cricetulus griseus CoA + octanoate
-
 ?
3.1.2.2 octanoyl-CoA + H2O
-
 Rattus norvegicus CoA + octanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O best substrate Cricetulus griseus CoA + tetradecanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + tetradecanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Cricetulus griseus CoA + tetradecanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
 ?
3.1.2.20 acyl-CoA + H2O
-
 Cricetulus griseus CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O
-
 Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Cricetulus griseus CoA + decanoate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Rattus norvegicus CoA + decanoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O
-
 Cricetulus griseus CoA + dodecanoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O
-
 Rattus norvegicus CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Cricetulus griseus CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O
-
 Cricetulus griseus CoA + octadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O
-
 Rattus norvegicus CoA + octadecanoate
-
 ?
3.1.2.20 octanoyl-CoA + H2O
-
 Cricetulus griseus CoA + octanoate
-
 ?
3.1.2.20 octanoyl-CoA + H2O
-
 Rattus norvegicus CoA + octanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O best substrate Cricetulus griseus CoA + tetradecanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + tetradecanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Cricetulus griseus CoA + tetradecanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 ? x * 40000, SDS-PAGE Rattus norvegicus
3.1.2.20 ? x * 40000, SDS-PAGE Rattus norvegicus