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Literature summary extracted from

  • Gross, R.W.
    Purification and characterization of mitochondrial thioesterase from rabbit myocardium and its inhibition by palmitoyl carnitine (1984), Biochim. Biophys. Acta, 802, 197-202.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 Ca2+
-
 Oryctolagus cuniculus
3.1.2.2 L-palmitoyl carnitine competitively inhibits at 0.02-04 mM Oryctolagus cuniculus
3.1.2.20 Ca2+
-
 Oryctolagus cuniculus
3.1.2.20 L-palmitoyl carnitine competitively inhibits at 0.02-04 mM Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 mitochondrion
-
 Oryctolagus cuniculus 5739
-
3.1.2.20 mitochondrion
-
 Oryctolagus cuniculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.2 Mg2+
-
 Oryctolagus cuniculus
3.1.2.20 Mg2+
-
 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 43000
-
 4 * 43000, SDS-PAGE Oryctolagus cuniculus
3.1.2.2 170000
-
 gel filtration Oryctolagus cuniculus
3.1.2.20 43000
-
 4 * 43000, SDS-PAGE Oryctolagus cuniculus
3.1.2.20 170000
-
 gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Oryctolagus cuniculus
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Oryctolagus cuniculus
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Oryctolagus cuniculus
-
-
-
3.1.2.20 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 705fold Oryctolagus cuniculus
3.1.2.20 705fold Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 heart
-
 Oryctolagus cuniculus
-
3.1.2.2 myocardium
-
 Oryctolagus cuniculus
-
3.1.2.20 heart
-
 Oryctolagus cuniculus
-
3.1.2.20 myocardium
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 1.8
-
-
 Oryctolagus cuniculus
3.1.2.20 1.8
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Oryctolagus cuniculus CoA + hexadecanoate
-
 ?
3.1.2.2 n-butyryl-CoA + H2O
-
 Oryctolagus cuniculus CoA + n-butanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Oryctolagus cuniculus CoA + oleate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Oryctolagus cuniculus CoA + palmitate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Oryctolagus cuniculus CoA + hexadecanoate
-
 ?
3.1.2.20 n-butyryl-CoA + H2O
-
 Oryctolagus cuniculus CoA + n-butanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Oryctolagus cuniculus CoA + oleate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Oryctolagus cuniculus CoA + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 tetramer 4 * 43000, SDS-PAGE Oryctolagus cuniculus
3.1.2.20 tetramer 4 * 43000, SDS-PAGE Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8
-
-
 Oryctolagus cuniculus
3.1.2.20 8
-
-
 Oryctolagus cuniculus