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Literature summary extracted from

  • Joyard, J.; Stumpf, P.K.
    Characterization of an acyl-coenzyme A thioesterase associated with the envelope of spinach chloroplasts (1980), Plant Physiol., 65, 1039-1043.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 bovine serum albumin
-
 Spinacia oleracea
3.1.2.20 bovine serum albumin
-
 Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 Ca2+ 10 mM inhibits the enzyme by 30-35% Spinacia oleracea
3.1.2.2 Mg2+ 10 mM inhibits the enzyme by 30-35% Spinacia oleracea
3.1.2.2 oleic acid 1 mM inhibits by 90% Spinacia oleracea
3.1.2.2 palmitic acid 1 mM inhibits by 30% Spinacia oleracea
3.1.2.2 unsaturated fatty acids
-
 Spinacia oleracea
3.1.2.20 Ca2+ 10 mM inhibits the enzyme by 30-35% Spinacia oleracea
3.1.2.20 Mg2+ 10 mM inhibits the enzyme by 30-35% Spinacia oleracea
3.1.2.20 oleic acid 1 mM inhibits by 90% Spinacia oleracea
3.1.2.20 palmitic acid 1 mM inhibits by 30% Spinacia oleracea
3.1.2.20 unsaturated fatty acids
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 chloroplast
-
 Spinacia oleracea 9507
-
3.1.2.2 peroxisome
-
 Spinacia oleracea 5777
-
3.1.2.20 chloroplast
-
 Spinacia oleracea 9507
-
3.1.2.20 peroxisome
-
 Spinacia oleracea 5777
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Spinacia oleracea
-
-
-
3.1.2.20 Spinacia oleracea
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -20°C over 1 month Spinacia oleracea
3.1.2.20 -20°C over 1 month Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 decanoyl-CoA + H2O
-
 Spinacia oleracea CoA + decanoate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Spinacia oleracea CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Spinacia oleracea CoA + hexadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Spinacia oleracea CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O just slowly hydrolyzed Spinacia oleracea CoA + oleate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Spinacia oleracea CoA + tetradecanoate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Spinacia oleracea CoA + decanoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Spinacia oleracea CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Spinacia oleracea CoA + hexadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Spinacia oleracea CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O just slowly hydrolyzed Spinacia oleracea CoA + oleate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Spinacia oleracea CoA + tetradecanoate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 9
-
-
 Spinacia oleracea
3.1.2.20 9
-
-
 Spinacia oleracea