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Literature summary extracted from

  • Berge, R.K.
    Purification and characterization of a long-chain acyl-CoA hydrolase from rat liver microsomes (1979), Biochim. Biophys. Acta, 574, 321-333.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 dithiothreitol
-
Rattus norvegicus
3.1.2.2 Triton X-100
-
Rattus norvegicus
3.1.2.20 dithiothreitol
-
Rattus norvegicus
3.1.2.20 Triton X-100
-
Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 acetyl-CoA
-
Rattus norvegicus
3.1.2.2 KCl
-
Rattus norvegicus
3.1.2.2 Mg2+ 0.5 mM gives 40% inhibition Rattus norvegicus
3.1.2.2 Mn2+ 0.2 mM gives 60% inhibition Rattus norvegicus
3.1.2.2 N-ethylmaleimide 0.001 mM gives 50% inhibition Rattus norvegicus
3.1.2.2 NaCl
-
Rattus norvegicus
3.1.2.2 p-hydroxymercuribenzoate 0.005 mM gives 50% inhibition Rattus norvegicus
3.1.2.20 acetyl-CoA
-
Rattus norvegicus
3.1.2.20 KCl
-
Rattus norvegicus
3.1.2.20 Mg2+ 0.5 mM gives 40% inhibition Rattus norvegicus
3.1.2.20 Mn2+ 0.2 mM gives 60% inhibition Rattus norvegicus
3.1.2.20 N-ethylmaleimide 0.001 mM gives 50% inhibition Rattus norvegicus
3.1.2.20 NaCl
-
Rattus norvegicus
3.1.2.20 p-hydroxymercuribenzoate 0.005 mM gives 50% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.0001
-
hexadecanoyl-CoA
-
Rattus norvegicus
3.1.2.20 0.0001
-
hexadecanoyl-CoA
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 microsome
-
Rattus norvegicus
-
-
3.1.2.20 microsome
-
Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 35000
-
x * 35000, SDS-PAGE Rattus norvegicus
3.1.2.20 35000
-
x * 35000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Rattus norvegicus
-
CoA + palmitate
-
?
3.1.2.20 palmitoyl-CoA + H2O Rattus norvegicus
-
CoA + palmitate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Rattus norvegicus
-
-
-
3.1.2.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2
-
Rattus norvegicus
3.1.2.20
-
Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.2.2
-
Rattus norvegicus
3.1.2.20
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 liver
-
Rattus norvegicus
-
3.1.2.20 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 1.15
-
-
Rattus norvegicus
3.1.2.20 1.15
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -20°C over 1 month Rattus norvegicus
3.1.2.2 -80°C for several months without notable loss of activity Rattus norvegicus
3.1.2.20 -20°C over 1 month Rattus norvegicus
3.1.2.20 -80°C for several months without notable loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
?
3.1.2.2 hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
?
3.1.2.2 oleoyl-CoA + H2O
-
Rattus norvegicus CoA + oleate
-
?
3.1.2.2 palmitoyl-CoA + H2O
-
Rattus norvegicus CoA + palmitate
-
?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
?
3.1.2.20 hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
?
3.1.2.20 oleoyl-CoA + H2O
-
Rattus norvegicus CoA + oleate
-
?
3.1.2.20 palmitoyl-CoA + H2O
-
Rattus norvegicus CoA + palmitate
-
?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 ? x * 35000, SDS-PAGE Rattus norvegicus
3.1.2.20 ? x * 35000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 6.9
-
-
Rattus norvegicus
3.1.2.2 7.5 8
-
Rattus norvegicus
3.1.2.2 7.8 8.2
-
Rattus norvegicus
3.1.2.20 6.9
-
-
Rattus norvegicus
3.1.2.20 7.5 8
-
Rattus norvegicus
3.1.2.20 7.8 8.2
-
Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 7.8 8.2
-
Rattus norvegicus
3.1.2.20 7.8 8.2
-
Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.2 0.001
-
N-ethylmaleimide
-
Rattus norvegicus
3.1.2.20 0.001
-
N-ethylmaleimide
-
Rattus norvegicus