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Literature summary extracted from
- Ubiquitin carboxy-terminal hydrolase L1 binds to and stabilizes monoubiquitin in neuron (2003), Hum. Mol. Genet., 12, 1945-1958.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.19.12 | isozyme L1: expression of wild-type and mutants in Escherichia coli as His-tagged proteins | Mus musculus |
| 3.4.19.12 | overexpression of isozyme L1 in mouse embryonic fibroblasts via adenovirus transfection | Mus musculus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.19.12 | C90S | site-directed mutagenesis, isozyme L1, inactive | Mus musculus |
| 3.4.19.12 | C90S | exchange of the active site cysteine | Mus musculus |
| 3.4.19.12 | D30K | site-directed mutagenesis, isozyme L1, inactive | Mus musculus |
| 3.4.19.12 | additional information | gad mouse null mutants of isozyme L1 show reduced monoubiquitin level in neurons, overexpression of the isozyme L1 leads to an increased monoubiquitin level | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.12 | ubiquitin | isozyme L1 | Mus musculus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.12 | 0.00016 | - |
ubiquitinyl-7-amido-4-methylcoumarin | 25°C | Mus musculus | |
| 3.4.19.12 | 0.00016 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant wild-type isozyme L1, pH 7.5, 25°C | Mus musculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.12 | additional information | Mus musculus | isozyme L1 associates and colocalizes with monoubiquitin and elongates ubiquitin half-life, null mutants show a reduced monoubiquitin level in neurons, while overexpression causes an increase in monoubiquitin level, mechanism | ? | - |
? | |
| 3.4.19.12 | additional information | Mus musculus | affects ubiquitin degradation and alters its metabolism. UCH-L1-mediated increases in ubiquitin levels are a function of UCH L1 affinity for ubiquitin rather than hydrolase activity. The enzyme insures ubiquitin stability within neurons | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | Mus musculus | - |
isozymes L1 and L3 | - |
| 3.4.19.12 | Mus musculus | Q9R0P9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.12 | recombinant His-tagged wild-type and mutants of isozyme L1 expressed in Escherichia coli | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | brain | isozymes L1, specific for neuronal cells, testis and ovary, and L3 | Mus musculus | - |
| 3.4.19.12 | additional information | no isozyme L1 expression in embryonic fibroblasts | Mus musculus | - |
| 3.4.19.12 | additional information | isozyme L3 is universally expressed in all tissues | Mus musculus | - |
| 3.4.19.12 | neuron | the enzyme insures ubiquitin stability within neurons | Mus musculus | - |
| 3.4.19.12 | ovary | isozyme L1, specific for neuronal cells, testis and ovary | Mus musculus | - |
| 3.4.19.12 | testis | isozyme L1, specific for neuronal cells, testis and ovary | Mus musculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.12 | additional information | isozyme L1 associates and colocalizes with monoubiquitin and elongates ubiquitin half-life, null mutants show a reduced monoubiquitin level in neurons, while overexpression causes an increase in monoubiquitin level, mechanism | Mus musculus | ? | - |
? | |
| 3.4.19.12 | additional information | affects ubiquitin degradation and alters its metabolism. UCH-L1-mediated increases in ubiquitin levels are a function of UCH L1 affinity for ubiquitin rather than hydrolase activity. The enzyme insures ubiquitin stability within neurons | Mus musculus | ? | - |
? | |
| 3.4.19.12 | ubiquitin-7-amido-4-methylcoumarin + H2O | recombinant wild-type isozyme L1 | Mus musculus | ubiquitin + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.19.12 | ubiquitinyl-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | ubiquitin + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.19.12 | ubiquitin carboxy-terminal hydrolase L1 | - |
Mus musculus |
| 3.4.19.12 | UCH | - |
Mus musculus |
| 3.4.19.12 | UCHL1 | - |
Mus musculus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 25 | - |
assay at | Mus musculus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.12 | 0.02 | - |
ubiquitinyl-7-amido-4-methylcoumarin | 25°C | Mus musculus | |
| 3.4.19.12 | 0.02 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant wild-type isozyme L1, pH 7.5, 25°C | Mus musculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 7.5 | - |
assay at | Mus musculus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.12 | 0.0033 | - |
ubiquitin | 25°C | Mus musculus | |
| 3.4.19.12 | 0.0033 | - |
ubiquitin | pH 7.5, 25°C, recombinant wild-type isozyme L1 | Mus musculus | |
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Release 2023.1 (January 2023)
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