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Literature summary extracted from

  • Kaitna, S.; Schnabel, H.; Schnabel, R.; Hyman, A.A.; Glotzer, M.
    A ubiquitin C-terminal hydrolase is required to maintain osmotic balance and execute actin-dependent processes in the early C. elegans embryo (2002), J. Cell Sci., 115, 2293-2302.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.12 co-expression of Doa4p with cyk-3 of Caenorhabditis elegans and ubiquitin-beta-galactosidase fusion protein in Escherichia coli Saccharomyces cerevisiae
3.4.19.12 gene cyk-3, chromosome mapping, co-expression of cyk-3 with Doa4p of Saccharomyces cerevisiae and ubiquitin-beta-galactosidase fusion protein in Escherichia coli Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
3.4.19.12 additional information
-
 Saccharomyces cerevisiae
3.4.19.12 additional information gene cyk-3 null mutants are lethal, mutants fail to polarize the actin cytoskeleton, to segregate germline determinants, to assemble an intact cleavage furrow, and are intrinsically defective in osmotic regulation and cytokinesis, the latter defect can be partially rescued by providing osmotic support Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.19.12 ubiquitin-protein + H2O Saccharomyces cerevisiae
-
 ubiquitin + protein
-
 ?
3.4.19.12 ubiquitin-protein + H2O Caenorhabditis elegans enzyme is crucial for polarization of the actin cytoskeleton, to segregate germline determinants, to assemble an intact cleavage furrow, for cytokinesis and to maintain osmotic balance and execute actin-dependent processes in the early embryo ubiquitin + protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.12 Caenorhabditis elegans
-
 gene cyk-3, defects are lethal
-
3.4.19.12 Saccharomyces cerevisiae
-
 Doa4p ubiquitin isopeptidase
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.12 embryo early stage Caenorhabditis elegans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.12 linear ubiquitin-beta-galactosidase + H2O recombinant fusion protein co-expressed with the enzyme in Escherichia coli Saccharomyces cerevisiae ubiquitin + protein
-
 ?
3.4.19.12 linear ubiquitin-beta-galactosidase + H2O recombinant fusion protein co-expressed with the enzyme in Escherichia coli Caenorhabditis elegans ubiquitin + protein
-
 ?
3.4.19.12 ubiquitin-protein + H2O
-
 Saccharomyces cerevisiae ubiquitin + protein
-
 ?
3.4.19.12 ubiquitin-protein + H2O enzyme is crucial for polarization of the actin cytoskeleton, to segregate germline determinants, to assemble an intact cleavage furrow, for cytokinesis and to maintain osmotic balance and execute actin-dependent processes in the early embryo Caenorhabditis elegans ubiquitin + protein
-
 ?